Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   I2439_RS00755 Genome accession   NZ_CP065191
Coordinates   129133..129567 (+) Length   144 a.a.
NCBI ID   WP_012677237.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain SEZ46     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 120242..133134 129133..129567 within 0


Gene organization within MGE regions


Location: 120242..133134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2439_RS00705 (HIEAAJJG_00142) - 120242..121288 (+) 1047 WP_196820074.1 acyltransferase family protein -
  I2439_RS00710 (HIEAAJJG_00143) - 121349..121714 (+) 366 WP_037580641.1 DUF1033 family protein -
  I2439_RS00715 (HIEAAJJG_00144) - 122247..122750 (+) 504 WP_196819761.1 hypothetical protein -
  I2439_RS00720 (HIEAAJJG_00145) - 123434..125437 (+) 2004 WP_196819762.1 DUF1430 domain-containing protein -
  I2439_RS00725 (HIEAAJJG_00146) - 125439..126086 (+) 648 WP_196819763.1 ATP-binding cassette domain-containing protein -
  I2439_RS00730 (HIEAAJJG_00147) comGA/cglA 126267..127205 (+) 939 WP_196819764.1 competence type IV pilus ATPase ComGA Machinery gene
  I2439_RS00735 (HIEAAJJG_00148) comYB 127138..128172 (+) 1035 WP_231191948.1 competence type IV pilus assembly protein ComGB Machinery gene
  I2439_RS00740 (HIEAAJJG_00149) comGC 128173..128496 (+) 324 Protein_112 competence type IV pilus major pilin ComGC -
  I2439_RS00745 (HIEAAJJG_00150) comGD 128474..128899 (+) 426 WP_196819766.1 competence type IV pilus minor pilin ComGD -
  I2439_RS00750 (HIEAAJJG_00151) comGE 128856..129146 (+) 291 WP_196819767.1 competence type IV pilus minor pilin ComGE -
  I2439_RS00755 (HIEAAJJG_00152) comYF 129133..129567 (+) 435 WP_012677237.1 competence type IV pilus minor pilin ComGF Machinery gene
  I2439_RS00760 (HIEAAJJG_00153) comGG 129545..129907 (+) 363 WP_012514785.1 competence type IV pilus minor pilin ComGG -
  I2439_RS00765 (HIEAAJJG_00154) comYH 129969..130922 (+) 954 WP_196819768.1 class I SAM-dependent methyltransferase Machinery gene
  I2439_RS00770 (HIEAAJJG_00155) - 130982..132181 (+) 1200 WP_165627047.1 acetate kinase -
  I2439_RS00775 (HIEAAJJG_00156) - 132544..133134 (-) 591 WP_196819769.1 hypothetical protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16155.70 Da        Isoelectric Point: 10.1988

>NTDB_id=507804 I2439_RS00755 WP_012677237.1 129133..129567(+) (comYF) [Streptococcus equi subsp. zooepidemicus strain SEZ46]
MKDSRLKAFTLIECLIALLVISGSLLVYQALTKSLMVSERYLAANDQDNWLLFSQQLRAELSGTTLQGVSNNRLYVEKDK
KTLSFGQVKSHDFRKAAGNGRGYQPMLFGLSSSQITAVGQQVIIKLKWQSGLERTFIYAFQEKG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=507804 I2439_RS00755 WP_012677237.1 129133..129567(+) (comYF) [Streptococcus equi subsp. zooepidemicus strain SEZ46]
TTGAAAGACAGTAGGTTAAAGGCTTTCACCTTGATAGAGTGCCTTATTGCCTTGCTTGTCATCTCAGGCTCTTTATTAGT
TTATCAGGCCTTAACCAAGAGCCTTATGGTGAGTGAGAGGTATCTAGCAGCAAATGATCAGGACAACTGGCTTTTGTTTT
CCCAGCAATTGCGAGCAGAGCTTTCAGGTACTACCTTACAGGGTGTCTCCAATAATAGGCTATATGTTGAGAAAGACAAG
AAAACTCTGTCCTTTGGACAGGTCAAAAGCCATGATTTTAGAAAAGCAGCTGGCAATGGTCGAGGCTATCAGCCCATGCT
GTTTGGCTTGTCAAGTAGCCAAATAACAGCAGTAGGTCAGCAGGTTATCATCAAGCTGAAATGGCAAAGCGGCTTAGAAA
GGACCTTTATTTATGCATTTCAAGAGAAGGGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

52.083

100

0.521
  comYF Streptococcus mutans UA159

51.389

100

0.514
  comGF/cglF Streptococcus mitis SK321

50.365

95.139

0.479
  comGF/cglF Streptococcus mitis NCTC 12261

49.635

95.139

0.472
  comGF Lactococcus lactis subsp. cremoris KW2

47.143

97.222

0.458
  comGF/cglF Streptococcus pneumoniae D39

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae R6

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae TIGR4

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae Rx1

48.175

95.139

0.458