Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I3J23_RS11540 Genome accession   NZ_CP065159
Coordinates   2222918..2223355 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2217918..2228355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS19720 - 2218498..2218659 (-) 162 Protein_2231 hypothetical protein -
  I3J23_RS11505 (I3J23_11505) - 2218776..2219978 (+) 1203 WP_025853783.1 GTP-binding protein -
  I3J23_RS11510 (I3J23_11510) - 2220043..2221179 (+) 1137 WP_048368274.1 zinc-dependent alcohol dehydrogenase -
  I3J23_RS11515 (I3J23_11515) - 2221194..2221628 (+) 435 WP_021494203.1 RDD family protein -
  I3J23_RS11520 (I3J23_11520) - 2221700..2222020 (+) 321 WP_015388719.1 YckD family protein -
  I3J23_RS11530 (I3J23_11530) - 2222239..2222457 (+) 219 Protein_2236 family 1 glycosylhydrolase -
  I3J23_RS11535 (I3J23_11535) nin/comJ 2222498..2222896 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  I3J23_RS11540 (I3J23_11540) nucA/comI 2222918..2223355 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  I3J23_RS11545 (I3J23_11545) hxlB 2223714..2224271 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  I3J23_RS11550 (I3J23_11550) hxlA 2224268..2224903 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  I3J23_RS11555 (I3J23_11555) - 2225135..2225497 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=507552 I3J23_RS11540 WP_003156588.1 2222918..2223355(-) (nucA/comI) [Bacillus amyloliquefaciens strain GXU-1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=507552 I3J23_RS11540 WP_003156588.1 2222918..2223355(-) (nucA/comI) [Bacillus amyloliquefaciens strain GXU-1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCAATAAAAGAAGGGCATTCCAGCATTTGCACCATTGATAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641