Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   I3J23_RS08100 Genome accession   NZ_CP065159
Coordinates   1563416..1564561 (+) Length   381 a.a.
NCBI ID   WP_088037228.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 1558416..1569561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS08080 (I3J23_08080) - 1558890..1559606 (-) 717 WP_048367767.1 Crp/Fnr family transcriptional regulator -
  I3J23_RS08085 (I3J23_08085) narK 1559704..1560894 (-) 1191 WP_044052814.1 nitrate transporter NarK -
  I3J23_RS08090 (I3J23_08090) argS 1561029..1562699 (-) 1671 WP_207579576.1 arginine--tRNA ligase -
  I3J23_RS08095 (I3J23_08095) - 1562696..1563124 (-) 429 WP_207579577.1 DUF1934 family protein -
  I3J23_RS08100 (I3J23_08100) rapF 1563416..1564561 (+) 1146 WP_088037228.1 tetratricopeptide repeat protein Regulator
  I3J23_RS08105 (I3J23_08105) - 1564545..1564664 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  I3J23_RS08110 (I3J23_08110) - 1564863..1565066 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  I3J23_RS08115 (I3J23_08115) - 1565080..1565283 (+) 204 WP_003151062.1 hypothetical protein -
  I3J23_RS19700 - 1565318..1565503 (+) 186 Protein_1599 DUF4177 domain-containing protein -
  I3J23_RS08120 (I3J23_08120) - 1565691..1565972 (+) 282 WP_014306021.1 hypothetical protein -
  I3J23_RS08125 (I3J23_08125) - 1566016..1566528 (+) 513 WP_032859656.1 stage II sporulation protein M -
  I3J23_RS08130 (I3J23_08130) - 1567119..1567337 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  I3J23_RS08135 (I3J23_08135) speB 1567507..1568379 (-) 873 WP_003151054.1 agmatinase -
  I3J23_RS08140 (I3J23_08140) speE 1568439..1569269 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45325.46 Da        Isoelectric Point: 4.9264

>NTDB_id=507541 I3J23_RS08100 WP_088037228.1 1563416..1564561(+) (rapF) [Bacillus amyloliquefaciens strain GXU-1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=507541 I3J23_RS08100 WP_088037228.1 1563416..1564561(+) (rapF) [Bacillus amyloliquefaciens strain GXU-1]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTATCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAAAGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.541

100

0.745
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53