Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I3F57_RS12300 Genome accession   NZ_CP065154
Coordinates   2543264..2544628 (-) Length   454 a.a.
NCBI ID   WP_003580798.1    Uniprot ID   -
Organism   Lacticaseibacillus paracasei subsp. tolerans strain ZY-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2538264..2549628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3F57_RS12270 (I3F57_12270) - 2538856..2538969 (-) 114 WP_003577835.1 bacteriocin -
  I3F57_RS15960 (I3F57_12275) - 2539013..2539117 (-) 105 WP_191994146.1 bacteriocin -
  I3F57_RS12280 (I3F57_12280) - 2539161..2539937 (-) 777 WP_003577837.1 ABC transporter permease -
  I3F57_RS12285 (I3F57_12285) - 2539941..2540846 (-) 906 WP_016366635.1 ATP-binding cassette domain-containing protein -
  I3F57_RS15965 - 2540867..2540998 (-) 132 WP_003580794.1 hypothetical protein -
  I3F57_RS12290 (I3F57_12290) - 2541127..2542023 (+) 897 WP_003580795.1 helix-turn-helix domain-containing protein -
  I3F57_RS12295 (I3F57_12295) - 2542127..2543242 (-) 1116 WP_003567252.1 PIN/TRAM domain-containing protein -
  I3F57_RS12300 (I3F57_12300) radA 2543264..2544628 (-) 1365 WP_003580798.1 DNA repair protein RadA Machinery gene
  I3F57_RS12305 (I3F57_12305) - 2544645..2545187 (-) 543 WP_003567256.1 dUTP diphosphatase -
  I3F57_RS12310 (I3F57_12310) - 2545409..2545699 (+) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  I3F57_RS12315 (I3F57_12315) - 2545814..2546191 (-) 378 WP_003567260.1 DUF805 domain-containing protein -
  I3F57_RS12320 (I3F57_12320) - 2546436..2547755 (+) 1320 WP_003571381.1 C1 family peptidase -
  I3F57_RS12325 (I3F57_12325) - 2547988..2549334 (+) 1347 WP_003576225.1 aminopeptidase C -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49140.50 Da        Isoelectric Point: 7.4216

>NTDB_id=507498 I3F57_RS12300 WP_003580798.1 2543264..2544628(-) (radA) [Lacticaseibacillus paracasei subsp. tolerans strain ZY-1]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKIKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=507498 I3F57_RS12300 WP_003580798.1 2543264..2544628(-) (radA) [Lacticaseibacillus paracasei subsp. tolerans strain ZY-1]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACCGCAGCCGGAAGCAAGA
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTATCAGGGCAGTTGGCTAATACCGGCGGAACGGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGCGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTATGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGCGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTTGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGACCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGAGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAACTGGGTTTCAAACGGATTTTTGTACCCCGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.212

99.78

0.681
  radA Streptococcus pneumoniae Rx1

68.212

99.78

0.681
  radA Streptococcus pneumoniae D39

68.212

99.78

0.681
  radA Streptococcus pneumoniae R6

68.212

99.78

0.681
  radA Streptococcus pneumoniae TIGR4

68.212

99.78

0.681
  radA Streptococcus mitis SK321

67.991

99.78

0.678
  radA Bacillus subtilis subsp. subtilis str. 168

64.537

100

0.645