Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BTD91_RS07625 Genome accession   NZ_CP065150
Coordinates   1744872..1746563 (+) Length   563 a.a.
NCBI ID   WP_046224652.1    Uniprot ID   -
Organism   Vibrio kanaloae strain R17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1739872..1751563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTD91_RS07605 (BTD91_07605) pilM 1742095..1743120 (+) 1026 WP_077680948.1 type IV pilus assembly protein PilM Machinery gene
  BTD91_RS07610 (BTD91_07610) pilN 1743104..1743703 (+) 600 WP_017055835.1 PilN domain-containing protein Machinery gene
  BTD91_RS07615 (BTD91_07615) pilO 1743696..1744307 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  BTD91_RS07620 (BTD91_07620) pilP 1744294..1744812 (+) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  BTD91_RS07625 (BTD91_07625) pilQ 1744872..1746563 (+) 1692 WP_046224652.1 type IV pilus secretin PilQ Machinery gene
  BTD91_RS07630 (BTD91_07630) aroK 1746740..1747258 (+) 519 WP_017061125.1 shikimate kinase AroK -
  BTD91_RS07635 (BTD91_07635) aroB 1747285..1748373 (+) 1089 WP_077680945.1 3-dehydroquinate synthase -
  BTD91_RS07640 (BTD91_07640) - 1748453..1749970 (+) 1518 WP_077680944.1 AAA family ATPase -
  BTD91_RS07645 (BTD91_07645) - 1750040..1750900 (+) 861 WP_077680943.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61285.82 Da        Isoelectric Point: 4.4054

>NTDB_id=507428 BTD91_RS07625 WP_046224652.1 1744872..1746563(+) (pilQ) [Vibrio kanaloae strain R17]
MNKGLQGLIVSVMLIFSVLSHAEDLPNKLENIDFRVNKNKDAVLIIELATSTVVVDVQRAQEGLSIELINTHVDDNKLQL
LDVKDFATLVEGVEVYNEAPSTRLLATISKDYQYEYNLKGRFIEVVISKPAIEEAVTEKSILEKEGKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREQQALEKSRLEEELG
ELKSEIIKIHFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRSIIESLDVPVKQVQIEARIVTV
TEGNLDELGVRWGASSTNGSFTVGGSIEGNYPSIAPYDSNGGNSVIDEYLNVNLGATSSNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=507428 BTD91_RS07625 WP_046224652.1 1744872..1746563(+) (pilQ) [Vibrio kanaloae strain R17]
ATGAACAAAGGGTTACAAGGCTTGATTGTGTCTGTGATGTTGATCTTTAGTGTGCTCAGTCACGCAGAGGACTTACCTAA
TAAGTTAGAAAATATTGATTTTAGGGTGAACAAAAATAAAGACGCTGTCCTAATTATCGAGCTCGCAACCAGCACGGTTG
TGGTGGATGTTCAACGTGCACAAGAGGGGCTAAGCATAGAGCTGATCAATACTCATGTTGATGACAACAAACTCCAGCTT
CTTGATGTGAAAGATTTCGCTACCTTGGTTGAAGGCGTGGAAGTGTATAACGAGGCCCCAAGCACTCGGTTGTTAGCGAC
AATCAGCAAGGATTACCAATACGAATACAATCTCAAAGGCCGTTTTATTGAGGTGGTGATCAGTAAACCTGCTATAGAAG
AAGCGGTAACAGAGAAAAGTATACTCGAGAAAGAGGGTAAGCTGATATCGATTAACTTCCAAGATATTCCAGTGCGAAAT
GTCTTGCAGCTTATCGCTGACTATAACGACTTTAACTTAGTCGTGTCTGATTCAGTAACCGGAAACCTAACTTTACGTTT
AGATGGTGTGCCCTGGCAGCAAGTTCTGGATATCATATTACAGGTCAAAGGGTTAGATAAGCGGGTAGATGGCAATGTCA
TTTTGGTAGCGCCTAAAGATGAACTCGACCTTCGAGAGCAGCAAGCGTTAGAGAAGTCGCGTTTAGAAGAGGAATTGGGT
GAGCTTAAGTCGGAAATTATTAAGATTCACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGAGGCGAGGGAGCGGT
GAGTATGTTGTCTGATCGGGGCTCGATTACTATTGATGAGCGCACTAATTCATTGCTTATTCGTGAACTGGAGGAAAACA
TTGCGGTGATACGAAGCATTATTGAATCTTTAGATGTCCCTGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTGACTGTT
ACTGAGGGGAACCTTGATGAGCTAGGAGTGCGTTGGGGAGCTTCTTCAACAAACGGAAGCTTCACGGTTGGTGGTTCTAT
CGAAGGCAACTACCCGTCTATTGCACCTTACGATAGTAATGGAGGGAATAGCGTCATTGATGAGTACCTCAACGTTAATT
TAGGTGCGACATCTTCAAATGCTTCGAGTATCGCATTTCAGGTTGCTAAACTGGGCTCTGATACTCTGCTCGACCTTGAG
TTATCAGCGTTACAACAAGAGTCAAAAGCTGAAATTATTTCGAGTCCACGTTTGATTACTACCAATAAAAAGCCCGCTTA
TATTGAGCAAGGTACTGAAATTCCTTATTTAGAGTCATCTTCAAGTGGTGCAACGTCAGTCGCATTTAAGAAAGCGGTAT
TGAGCCTTAAGGTGACGCCGCAGATCACGCCAGACAATCGTTTGGTGTTGGATTTGAGCGTAACTCAAGATAGGCCCGGA
CAGGTGGTGAAAACCGGAACTGGAGAGGCTGTTGCAATTGACACCCAGCGTATTGGCACGCAAGTGCTTGTTAATAATGG
TGAAACAGTTGTTCTTGGTGGGATATTTCAGCACAGTGTGAGTAGCACCGTCGATAAAGTTCCTCTATTGGGAGACCTGC
CTGTATTAGGGGCTTTGTTCCGCAGAAGCTACGAAAATGTAGGGAAAAGTGAGTTGTTGATCTTTGTTACACCGAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

78.938

96.98

0.766
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.938

96.98

0.766
  pilQ Vibrio campbellii strain DS40M4

79.19

96.448

0.764
  pilQ Legionella pneumophila strain Lp02

42.125

96.98

0.409
  pilQ Legionella pneumophila strain ERS1305867

42.125

96.98

0.409
  pilQ Pseudomonas aeruginosa PAK

40.827

98.757

0.403
  comQ Acinetobacter baylyi ADP1

39.286

99.467

0.391
  pilQ Acinetobacter baumannii D1279779

37.543

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.543

100

0.385