Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BTD91_RS07615 Genome accession   NZ_CP065150
Coordinates   1743696..1744307 (+) Length   203 a.a.
NCBI ID   WP_060468742.1    Uniprot ID   A0A120DG30
Organism   Vibrio kanaloae strain R17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1738696..1749307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTD91_RS07600 (BTD91_07600) - 1739382..1741943 (-) 2562 WP_175627321.1 penicillin-binding protein 1A -
  BTD91_RS07605 (BTD91_07605) pilM 1742095..1743120 (+) 1026 WP_077680948.1 type IV pilus assembly protein PilM Machinery gene
  BTD91_RS07610 (BTD91_07610) pilN 1743104..1743703 (+) 600 WP_017055835.1 PilN domain-containing protein Machinery gene
  BTD91_RS07615 (BTD91_07615) pilO 1743696..1744307 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  BTD91_RS07620 (BTD91_07620) pilP 1744294..1744812 (+) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  BTD91_RS07625 (BTD91_07625) pilQ 1744872..1746563 (+) 1692 WP_046224652.1 type IV pilus secretin PilQ Machinery gene
  BTD91_RS07630 (BTD91_07630) aroK 1746740..1747258 (+) 519 WP_017061125.1 shikimate kinase AroK -
  BTD91_RS07635 (BTD91_07635) aroB 1747285..1748373 (+) 1089 WP_077680945.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23477.94 Da        Isoelectric Point: 4.7445

>NTDB_id=507426 BTD91_RS07615 WP_060468742.1 1743696..1744307(+) (pilO) [Vibrio kanaloae strain R17]
MVSFQNRVSLQDLDVDEITEWPLLPQLLVIFALMILIQGVGTWLYIAPLDDELQQMKQQEQTLKTTLRIKANKAAVLPKL
QSQLDDLTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHAIGDFSAAIA
KLPRIISFNDVSWQRVSQESSTLHFRVRAYTYQFKPELSDETQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=507426 BTD91_RS07615 WP_060468742.1 1743696..1744307(+) (pilO) [Vibrio kanaloae strain R17]
ATGGTTAGTTTTCAAAATCGGGTGAGCTTGCAAGATCTCGATGTCGATGAGATCACCGAATGGCCTTTATTGCCTCAGCT
ATTGGTCATTTTTGCCTTGATGATACTTATCCAAGGTGTGGGTACTTGGTTGTATATTGCTCCTTTGGACGATGAACTTC
AGCAGATGAAACAGCAAGAGCAGACGCTTAAAACAACTTTGCGGATTAAAGCCAATAAGGCGGCGGTTTTGCCTAAGCTC
CAGAGTCAGTTGGATGATTTAACCAGTCGTTATGATTATCTATTAGAGCAGCTACCAGTACAAAAAGAGTTGGCAAGTAT
GTTGGCCTCAGTGAATGAGCTTGGGTTGGATAATAAGCTGACGTTTACGCGTATTGATTGGGGACAGAAGCAAAACAAGG
AGTTTCTCTATCGTTTACCTCTCAATATAGAGCTAACAGGGGACTATCACGCGATTGGAGACTTCTCTGCAGCCATCGCC
AAACTCCCACGAATCATTAGTTTTAATGATGTGAGTTGGCAGCGCGTCAGCCAAGAAAGTAGCACGCTCCATTTCAGGGT
GCGAGCGTATACCTACCAATTTAAGCCGGAGTTGAGTGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A120DG30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

73.711

95.567

0.704
  pilO Vibrio campbellii strain DS40M4

70.37

93.103

0.655