Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I0K14_RS20140 Genome accession   NZ_CP065149
Coordinates   3926582..3927625 (-) Length   347 a.a.
NCBI ID   WP_001013206.1    Uniprot ID   -
Organism   Bacillus paranthracis strain Gxun-30     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3921582..3932625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0K14_RS20095 (I0K14_20095) - 3922010..3922210 (-) 201 WP_000106079.1 YqzE family protein -
  I0K14_RS20100 (I0K14_20100) - 3922249..3922746 (-) 498 WP_000836627.1 shikimate kinase -
  I0K14_RS20105 (I0K14_20105) - 3922866..3923516 (-) 651 WP_000183848.1 2OG-Fe(II) oxygenase -
  I0K14_RS20110 (I0K14_20110) comGG 3923692..3924063 (-) 372 WP_000595196.1 competence type IV pilus minor pilin ComGG -
  I0K14_RS20115 (I0K14_20115) comGF 3924060..3924530 (-) 471 WP_000923058.1 competence type IV pilus minor pilin ComGF -
  I0K14_RS20120 (I0K14_20120) comGE 3924500..3924802 (-) 303 WP_000829518.1 competence type IV pilus minor pilin ComGE -
  I0K14_RS20125 (I0K14_20125) comGD 3924795..3925250 (-) 456 WP_000810398.1 comG operon protein ComGD -
  I0K14_RS20130 (I0K14_20130) comGC 3925247..3925546 (-) 300 WP_001178694.1 competence type IV pilus major pilin ComGC -
  I0K14_RS20135 (I0K14_20135) comGB 3925558..3926589 (-) 1032 WP_000473554.1 competence type IV pilus assembly protein ComGB -
  I0K14_RS20140 (I0K14_20140) comGA 3926582..3927625 (-) 1044 WP_001013206.1 competence protein ComGA Machinery gene
  I0K14_RS20145 (I0K14_20145) - 3927831..3928526 (+) 696 WP_000434109.1 helix-turn-helix transcriptional regulator -
  I0K14_RS20150 (I0K14_20150) - 3928652..3928894 (+) 243 WP_000440714.1 DUF2626 domain-containing protein -
  I0K14_RS20155 (I0K14_20155) - 3929002..3930396 (+) 1395 WP_001094339.1 L-cystine transporter -
  I0K14_RS20160 (I0K14_20160) - 3930571..3930978 (-) 408 WP_000877912.1 hypothetical protein -
  I0K14_RS20165 (I0K14_20165) - 3931066..3931281 (-) 216 WP_001008331.1 DUF3912 family protein -
  I0K14_RS20170 (I0K14_20170) - 3931536..3931847 (+) 312 WP_001093243.1 hypothetical protein -
  I0K14_RS20175 (I0K14_20175) - 3931884..3932372 (-) 489 WP_000764271.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39361.84 Da        Isoelectric Point: 9.1826

>NTDB_id=507392 I0K14_RS20140 WP_001013206.1 3926582..3927625(-) (comGA) [Bacillus paranthracis strain Gxun-30]
MNGIESFANTILKEACRVQASDLHIVPRKKDVVIQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=507392 I0K14_RS20140 WP_001013206.1 3926582..3927625(-) (comGA) [Bacillus paranthracis strain Gxun-30]
ATGAATGGAATTGAAAGTTTTGCGAATACGATTTTGAAAGAGGCGTGTAGGGTACAAGCTTCGGATTTACATATTGTGCC
CCGGAAGAAGGATGTAGTGATTCAACTGCGCATAGGGAAAGATTTAATGACGAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTACGTCTTTCCACGCTTCCAACTGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGGTTACTCGTATTTACTGGACCGACTGGATCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTGG
GTGAAATTCGTGATGAAGAAACAGCAAAAATCGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGAGCAATACTTCGGTTTATGGACTTTGGCATAACAAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTTGAATTGAAGTGTCCGTTTTGTAAAAGAAAGTGCTCAACTGTATGTAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACGTACAAGCATGAGACATTAGAGTCTTCGTTGCGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.501

100

0.585
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366