Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I0K14_RS18925 Genome accession   NZ_CP065149
Coordinates   3718516..3718950 (-) Length   144 a.a.
NCBI ID   WP_000811506.1    Uniprot ID   -
Organism   Bacillus paranthracis strain Gxun-30     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3713516..3723950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0K14_RS18905 (I0K14_18905) - 3713591..3714379 (+) 789 WP_002163529.1 RNA-guided endonuclease TnpB family protein -
  I0K14_RS18910 (I0K14_18910) - 3714539..3715096 (+) 558 WP_002163528.1 PadR family transcriptional regulator -
  I0K14_RS18915 (I0K14_18915) metE 3715764..3718052 (+) 2289 WP_001007597.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  I0K14_RS18920 (I0K14_18920) comJ 3718090..3718479 (-) 390 WP_000424017.1 competence protein ComJ -
  I0K14_RS18925 (I0K14_18925) nucA/comI 3718516..3718950 (-) 435 WP_000811506.1 DNA-entry nuclease Machinery gene
  I0K14_RS18930 (I0K14_18930) - 3719187..3719936 (+) 750 WP_000388460.1 DUF3967 domain-containing protein -
  I0K14_RS18935 (I0K14_18935) kinB 3719990..3721264 (-) 1275 WP_000420385.1 sporulation sensor histidine kinase KinB -
  I0K14_RS18940 (I0K14_18940) - 3721574..3721774 (+) 201 WP_000929285.1 hypothetical protein -
  I0K14_RS18945 (I0K14_18945) - 3721898..3723061 (+) 1164 WP_000434564.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16024.36 Da        Isoelectric Point: 8.4353

>NTDB_id=507389 I0K14_RS18925 WP_000811506.1 3718516..3718950(-) (nucA/comI) [Bacillus paranthracis strain Gxun-30]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPRDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=507389 I0K14_RS18925 WP_000811506.1 3718516..3718950(-) (nucA/comI) [Bacillus paranthracis strain Gxun-30]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAGAGATCGATACCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTGTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCACATCGAATATATAAGTCCAGCAGATAATCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTAAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556