Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   IRJ20_RS00650 Genome accession   NZ_CP065137
Coordinates   96883..97491 (+) Length   202 a.a.
NCBI ID   WP_015417702.1    Uniprot ID   -
Organism   Bacillus sp. A1(2020)     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 91883..102491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ20_RS00610 - 92935..93441 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  IRJ20_RS00615 - 93580..93993 (+) 414 WP_039254739.1 hypothetical protein -
  IRJ20_RS00620 - 94023..94457 (-) 435 WP_007409493.1 YppG family protein -
  IRJ20_RS00625 - 94644..94826 (+) 183 WP_003153532.1 YppF family protein -
  IRJ20_RS00630 - 94865..95233 (-) 369 WP_007409492.1 YppE family protein -
  IRJ20_RS00635 - 95279..95524 (-) 246 WP_012117836.1 DUF5446 family protein -
  IRJ20_RS00640 sspM 95731..95835 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  IRJ20_RS00645 - 95879..96841 (-) 963 WP_015417701.1 DUF2515 domain-containing protein -
  IRJ20_RS00650 recU 96883..97491 (+) 609 WP_015417702.1 Holliday junction resolvase RecU Machinery gene
  IRJ20_RS00655 - 97530..100313 (+) 2784 WP_039254735.1 PBP1A family penicillin-binding protein -
  IRJ20_RS00660 - 100391..100882 (-) 492 WP_039254733.1 YpoC family protein -
  IRJ20_RS00665 nth 100879..101538 (-) 660 WP_007612187.1 endonuclease III -
  IRJ20_RS00670 dnaD 101557..102255 (-) 699 WP_003153516.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23675.77 Da        Isoelectric Point: 9.5779

>NTDB_id=507189 IRJ20_RS00650 WP_015417702.1 96883..97491(+) (recU) [Bacillus sp. A1(2020)]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLHNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
NNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=507189 IRJ20_RS00650 WP_015417702.1 96883..97491(+) (recU) [Bacillus sp. A1(2020)]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCCCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCCACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTTGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCATAATTTTCACGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AACAATGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

89.95

98.515

0.886