Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I2434_RS01315 Genome accession   NZ_CP065058
Coordinates   254550..255911 (+) Length   453 a.a.
NCBI ID   WP_012514884.1    Uniprot ID   B4U0R5
Organism   Streptococcus equi subsp. zooepidemicus strain SEZ18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 249550..260911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2434_RS01300 (MCPGFBBE_00257) - 251132..252928 (+) 1797 WP_165623545.1 ABC transporter ATP-binding protein -
  I2434_RS01305 (MCPGFBBE_00258) - 253090..253857 (+) 768 WP_012678598.1 epoxyqueuosine reductase QueH -
  I2434_RS01310 (MCPGFBBE_00259) - 254037..254483 (+) 447 WP_012678597.1 dUTP diphosphatase -
  I2434_RS01315 (MCPGFBBE_00260) radA 254550..255911 (+) 1362 WP_012514884.1 DNA repair protein RadA Machinery gene
  I2434_RS01320 (MCPGFBBE_00261) - 256072..256350 (-) 279 WP_041790617.1 hypothetical protein -
  I2434_RS01325 (MCPGFBBE_00262) - 256734..257240 (+) 507 WP_012678595.1 carbonic anhydrase -
  I2434_RS01330 (MCPGFBBE_00263) - 257368..258081 (+) 714 WP_042671341.1 TIGR00266 family protein -
  I2434_RS01335 (MCPGFBBE_00264) gltX 258689..260134 (+) 1446 WP_043029311.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49333.77 Da        Isoelectric Point: 6.7545

>NTDB_id=506407 I2434_RS01315 WP_012514884.1 254550..255911(+) (radA) [Streptococcus equi subsp. zooepidemicus strain SEZ18]
MAKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLKHVNGVRYQRIQTEMNEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLYVSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIE
AIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQALVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKLYAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=506407 I2434_RS01315 WP_012514884.1 254550..255911(+) (radA) [Streptococcus equi subsp. zooepidemicus strain SEZ18]
ATAGCTAAGAAAAAGGCGAGGTTTATCTGTGGAGAATGTGGCTATCAGTCACCCAAATATCTGGGGCGCTGCCCTAATTG
CTCTGCTTGGTCTTCCTTTGTAGAGGAGACAGAGGTCAAGGACGTCAAAAATGCCCGTATTAGCCTGACTGGAGAAAAGA
GTAAGCCCATCAAGCTAAAGCATGTCAATGGCGTTCGCTACCAGCGTATCCAGACTGAGATGAATGAGTTCAATCGTGTG
CTGGGCGGAGGTGTTGTACCGGGGAGCTTGGTTTTGATTGGTGGTGATCCTGGTATTGGTAAGTCAACGCTGCTCTTGCA
GGTTTCTATCCAGCTTGCGGATAAGGGAACTGTTTTATATGTCTCTGGAGAGGAATCGGCAGAGCAGATCAAGATGCGCA
GTGATCGTTTGGGACAGACCGACAATGATTTTTACCTCTATGCGGAGACCAATATGCAGGCTATTCGGGCAGAGATTGAA
GCCATTCAGCCGGATTTTTTGATTATTGATTCTATTCAGACGATCATGAGTCCGGAGATTTCGGGTGTTCAGGGCTCGGT
CAGTCAGGTGAGAGAGGTTACTGCCGAATTGCTGCAGCTTGCAAAGTCTAATCATATCGCTACCTTTATCGTGGGCCATG
TGACCAAGGAGGGGACCCTAGCAGGGCCCCGCATGCTGGAGCATATGGTTGATACTGTGCTTTATTTTGAAGGGGAAAGA
CACCACACCTTCCGAATTCTGCGAGCTGTTAAAAACCGTTTTGGCTCAACCAATGAGATTGGTATTTTTGACATGCAATC
AGGCGGTCTGGTGGAGGTTCTCAACCCCAGTCAGGTTTTCTTAGAGGAGCGTCTGGACGGCGCAACAGGCTCGGCTGTTG
TCGTTACAATGGAGGGCAGTAGGCCAATTTTAGCTGAGGTGCAGGCCTTGGTGACACCGACTGTTTTTGGCAATGCTAGA
CGAACCACTACTGGATTAGACTTTAATCGTGTTAGCCTGATTATGGCTGTCCTAGAAAAGCGCTGCGGCCTGCTGCTACA
AAATCAAGATGCCTACCTCAAATCAGCAGGAGGTGTGAAGCTAGATGAGCCTGCTATTGATTTGGCAGTTGCTGTGGCTA
TTGCTTCTAGCTACAAGGAAAAACCAACCAACCCTCAGGAGGCCTTCTTAGGAGAGATTGGCTTGACGGGCGAGATTCGG
CGGGTCACTCGTATTGAGCAGCGGATCAATGAGGCTGCAAAGCTTGGCTTTACCAAGCTTTACGCTCCTAAAAGCTCATT
GCAGGGCATTGACATACCAAGTGGGATTGAGGTTGTTGGTGTAATGACAGTAGGAGAGGTCTTAAAGGCAGTCTTTGCTT
AG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4U0R5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

84.327

100

0.843
  radA Streptococcus pneumoniae D39

84.327

100

0.843
  radA Streptococcus pneumoniae R6

84.327

100

0.843
  radA Streptococcus pneumoniae TIGR4

84.327

100

0.843
  radA Streptococcus mitis SK321

84.327

100

0.843
  radA Streptococcus mitis NCTC 12261

84.106

100

0.841
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611