Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IV454_RS28170 Genome accession   NZ_CP065053
Coordinates   6420143..6421519 (-) Length   458 a.a.
NCBI ID   WP_206088846.1    Uniprot ID   -
Organism   Massilia antarctica strain P8398     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 6415143..6426519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IV454_RS28145 (IV454_28135) - 6415781..6416014 (+) 234 WP_206088841.1 helix-turn-helix transcriptional regulator -
  IV454_RS28150 (IV454_28140) - 6416467..6416808 (+) 342 WP_206088842.1 gamma-glutamylcyclotransferase family protein -
  IV454_RS28155 (IV454_28145) - 6416862..6418232 (-) 1371 WP_206088843.1 glutamine synthetase family protein -
  IV454_RS28160 (IV454_28150) fliD 6418244..6419611 (-) 1368 WP_206088844.1 flagellar filament capping protein FliD -
  IV454_RS28165 (IV454_28155) - 6419762..6420082 (-) 321 WP_282961383.1 PilZ domain-containing protein -
  IV454_RS28170 (IV454_28160) radA 6420143..6421519 (-) 1377 WP_206088846.1 DNA repair protein RadA Machinery gene
  IV454_RS28175 (IV454_28165) alr 6421512..6422618 (-) 1107 WP_206092878.1 alanine racemase -
  IV454_RS28180 (IV454_28170) lplT 6422746..6424008 (+) 1263 WP_206088847.1 lysophospholipid transporter LplT -
  IV454_RS28185 (IV454_28175) - 6424009..6424968 (-) 960 WP_206088848.1 DUF1853 family protein -
  IV454_RS28190 (IV454_28180) - 6424952..6425827 (-) 876 WP_206088849.1 uracil-DNA glycosylase -
  IV454_RS28195 (IV454_28185) rimI 6425824..6426294 (-) 471 WP_054263167.1 ribosomal protein S18-alanine N-acetyltransferase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48735.23 Da        Isoelectric Point: 7.4345

>NTDB_id=506273 IV454_RS28170 WP_206088846.1 6420143..6421519(-) (radA) [Massilia antarctica strain P8398]
MAKAKTQYTCSDCGGISSKWTGQCTACHQWNTMVETVVDTPGVNRLSQTQHKSLAQTAPVLSLADIEAVDVPRFGTGIEE
FDRVLGGGLVSGGVVLIGGDPGIGKSTLLLQALANISHSKSTLYVSGEESGAQIALRARRLGVDGKELKLQAEIQLEKIL
GTLADLKPEVAVIDSIQTVYSDALTSAPGSVAQVRECAAQLTRVAKQTGVTIILVGHVTKEGALAGPRVLEHIVDTVLYF
EGDPQSSFRLVRAIKNRFGAVNELGVFAMTEKGLKGVSNPSALFLSQHDNQVPGSCVMVTQEGTRPLLVEIQALVDTSHL
PNARRLSVGLEQNRLAMLLAVLHRHAGIAAFDQDVFINAVGGVKITEPAADLAVLLAINSSMRSKPLPRGLVVFGEVGLA
GEIRPAPRGQERLREAAKLGFSIAMIPKANVPKQKIEGMTIVAVERIDEAFNKLRELD

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=506273 IV454_RS28170 WP_206088846.1 6420143..6421519(-) (radA) [Massilia antarctica strain P8398]
ATGGCTAAAGCTAAAACGCAATACACCTGCAGCGACTGCGGCGGGATCAGCAGCAAATGGACGGGGCAGTGCACGGCTTG
CCATCAGTGGAACACGATGGTCGAGACGGTGGTCGACACGCCGGGTGTGAACCGCCTTTCGCAAACCCAGCACAAGAGCC
TGGCGCAGACAGCGCCGGTGTTGTCGCTGGCCGACATCGAGGCGGTCGACGTGCCGCGTTTCGGCACCGGCATCGAGGAG
TTCGACCGCGTGCTGGGCGGCGGCCTGGTGTCGGGCGGGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAATCGAC
CCTGCTGCTGCAGGCCTTGGCCAATATCTCGCATAGTAAGAGTACCTTGTATGTGAGCGGCGAGGAATCGGGCGCCCAGA
TCGCCCTGCGCGCGCGCCGCCTCGGGGTCGATGGCAAGGAACTTAAACTGCAGGCCGAGATCCAACTCGAGAAGATCCTC
GGGACCCTGGCCGACCTGAAGCCCGAGGTGGCGGTGATCGACTCGATCCAGACCGTGTATTCCGACGCGCTCACCTCGGC
GCCCGGTTCGGTGGCGCAGGTGCGCGAGTGCGCCGCCCAGCTCACGCGGGTGGCCAAGCAGACCGGGGTGACCATCATCC
TGGTTGGCCACGTGACCAAAGAGGGTGCGCTGGCCGGTCCCCGCGTGCTGGAACATATTGTCGATACGGTGCTGTATTTC
GAGGGCGATCCCCAGTCCAGTTTCCGGCTGGTGCGGGCCATCAAGAACCGTTTCGGCGCGGTGAACGAGCTGGGCGTGTT
CGCGATGACGGAAAAAGGACTGAAAGGGGTGTCCAACCCGTCGGCGCTGTTTTTGTCGCAGCACGACAACCAGGTGCCGG
GTTCGTGCGTGATGGTGACGCAGGAGGGCACGCGCCCCTTGCTGGTCGAAATCCAGGCGCTGGTCGATACCAGCCACCTG
CCCAATGCGCGGCGCCTGTCGGTGGGGCTGGAGCAAAACCGGCTGGCGATGCTGCTGGCCGTGCTGCACCGGCACGCCGG
CATCGCCGCCTTCGACCAGGATGTCTTCATCAATGCGGTGGGCGGGGTCAAGATCACCGAACCGGCGGCCGACCTGGCGG
TACTGCTGGCGATTAACTCGTCGATGCGCAGTAAGCCATTGCCGCGCGGGCTGGTGGTGTTTGGTGAAGTGGGGCTGGCG
GGCGAAATCCGGCCGGCACCGCGCGGCCAGGAACGCTTGCGCGAAGCGGCCAAGCTGGGTTTTTCAATCGCGATGATTCC
CAAGGCGAATGTGCCGAAGCAAAAAATCGAAGGCATGACCATTGTCGCGGTCGAGCGTATCGATGAGGCATTTAATAAAC
TGCGCGAGCTCGATTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(361-452)

AAA domain (AAA_25)

(66-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.352

99.345

0.48
  radA Streptococcus pneumoniae Rx1

47.072

100

0.474
  radA Streptococcus pneumoniae D39

47.072

100

0.474
  radA Streptococcus pneumoniae R6

47.072

100

0.474
  radA Streptococcus pneumoniae TIGR4

47.072

100

0.474
  radA Streptococcus mitis SK321

47.072

100

0.474
  radA Streptococcus mitis NCTC 12261

47.072

100

0.474