Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MY9_RS00585 Genome accession   NC_017743
Coordinates   106152..107528 (+) Length   458 a.a.
NCBI ID   WP_014662515.1    Uniprot ID   -
Organism   Bacillus sp. JS     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101152..112528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY9_RS00565 (MY9_0082) ctsR 101505..101969 (+) 465 WP_014662512.1 transcriptional regulator CtsR -
  MY9_RS00570 (MY9_0083) mcsA 101983..102540 (+) 558 WP_041521234.1 protein-arginine kinase activator protein McsA -
  MY9_RS00575 (MY9_0084) - 102540..103631 (+) 1092 WP_014662514.1 protein arginine kinase -
  MY9_RS00580 (MY9_0085) clpC 103628..106060 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MY9_RS00585 (MY9_0086) radA 106152..107528 (+) 1377 WP_014662515.1 DNA repair protein RadA Machinery gene
  MY9_RS00590 (MY9_0087) disA 107532..108614 (+) 1083 WP_014662516.1 DNA integrity scanning diadenylate cyclase DisA -
  MY9_RS00595 (MY9_0088) - 108730..109830 (+) 1101 WP_014662517.1 PIN/TRAM domain-containing protein -
  MY9_RS00600 (MY9_0089) ispD 109845..110543 (+) 699 WP_014662518.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MY9_RS00605 (MY9_0090) ispF 110536..111012 (+) 477 WP_014475594.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49454.94 Da        Isoelectric Point: 8.2399

>NTDB_id=50575 MY9_RS00585 WP_014662515.1 106152..107528(+) (radA) [Bacillus sp. JS]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSSQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=50575 MY9_RS00585 WP_014662515.1 106152..107528(+) (radA) [Bacillus sp. JS]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTTGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTTAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATCGGCGGTGATCCTGGTATCGGAAAGTCAACGTT
ATTACTTCAGGTTTCCTCTCAATTATCAGGCTCATCAAACAGTGTGCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTTCGAGCTGACCGCCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTATACCAAAGCGATATCACATCGGC
ACCGGGCAGCGTTTCACAGGTCAGAGAATGTACCGCTGAGCTCATGAAAATTGCAAAAACAAAAGGTATCCCGATTTTTA
TCGTGGGACACGTGACGAAAGAAGGGTCTATTGCAGGGCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGTCACCATACTTTCCGCATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATATT
TGAAATGCGGGAAGAGGGGCTGACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAGGAACGCTCTGCGGGTTCCGCGG
GCTCGAGTATCACTGCTTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGAAATCCAAGGCGTATGGCAACGGGAATAGACCACAACAGGGTTTCTCTGTTAATGGCTGTATTAGAAAAAAGAGTGGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATCAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTGCGACGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCTAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment