Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I1H34_RS23585 Genome accession   NZ_CP064923
Coordinates   5173726..5175753 (+) Length   675 a.a.
NCBI ID   WP_212663327.1    Uniprot ID   -
Organism   Acaryochloris marina S15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5168726..5180753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I1H34_RS23570 (I1H34_23530) - 5169282..5170391 (+) 1110 WP_212663324.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
  I1H34_RS23575 (I1H34_23535) - 5170396..5171736 (-) 1341 WP_212663325.1 TldD/PmbA family protein -
  I1H34_RS23580 (I1H34_23540) - 5171817..5173286 (-) 1470 WP_212663326.1 TldD/PmbA family protein -
  I1H34_RS23585 (I1H34_23545) pilB/pilB1 5173726..5175753 (+) 2028 WP_212663327.1 GspE/PulE family protein Machinery gene
  I1H34_RS23590 (I1H34_23550) - 5175798..5176973 (+) 1176 WP_212663328.1 glycosyltransferase family 4 protein -
  I1H34_RS23595 (I1H34_23555) - 5177075..5177692 (-) 618 WP_212663329.1 50S ribosomal protein L25/general stress protein Ctc -
  I1H34_RS23600 (I1H34_23560) - 5178061..5179236 (-) 1176 WP_212663330.1 glycosyltransferase family 4 protein -
  I1H34_RS23605 (I1H34_23565) - 5179319..5180236 (+) 918 WP_212663331.1 hypothetical protein -

Sequence

Protein


Download         Length: 675 a.a.        Molecular weight: 75369.66 Da        Isoelectric Point: 5.0388

>NTDB_id=505667 I1H34_RS23585 WP_212663327.1 5173726..5175753(+) (pilB/pilB1) [Acaryochloris marina S15]
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPLPPRLLRQIKRQQLFELKILY
GLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLACNDDVDPPVVRLGMVNPDHLSAIDDVDYLLRSNGMTFQRVV
LDLDTFQMLIAKFLDAQAKRQATIQENLPVEYGLEDDLDRLGGIEDLSTNDADINLDEALLDENAAPVISLVNKILAKAL
QNDISDIHIEPQEDNLRVRFRKDGLLHNAFAPLPRRIAPALVSRFKIIANLDIAERRLPQDGRILRLFQGRKVDFRVSTL
PSRYGEKVVLRILDNSAMQLGLNNLIDDEASLEIVREMVARPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDP
IEYTLQGITQVQVLREKGMDFASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEVTFYKANTSTSDDILATANTEDACPTCAGIG
YSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQTLLAYSLNLVEKGATSLEEVERVTFSDSGLKTELKAKRKT
ALNCLVCNAGLLPEWLDCPYCLTPRFAEHNPANQL

Nucleotide


Download         Length: 2028 bp        

>NTDB_id=505667 I1H34_RS23585 WP_212663327.1 5173726..5175753(+) (pilB/pilB1) [Acaryochloris marina S15]
ATGGAGAATAACTCATCCAAGCGGGAACTGCTCGTTAGAAACAATTTTTCTCCTTCAAACGAAAAATTAATCAAGAGTGG
CTATGTAGATCAAGCACGCTTGCGAGAAGCGATTTATGAAAGTCGGCGAAAGGGACGCTCGCTACTAGCGATTCTCCAGA
AGATGACGGGGAAACCTTTGCCTCCCCGGCTTCTCCGTCAGATTAAGCGTCAGCAGCTATTTGAACTCAAAATTCTCTAT
GGGCTGGATTGCCTCGATCCTGAGCTCACCCAGGTTGACTTTGACGAAATTGATCGACTGATTCGCACGGTCATCCCCCT
CGACTATTGTCGTCGCTATCAGATTTTGCCCCTAGCCTGCAATGATGATGTCGATCCACCTGTTGTCCGGCTGGGGATGG
TCAACCCAGATCACCTCAGTGCCATTGATGATGTTGACTATTTGCTGCGCTCCAATGGAATGACCTTTCAGCGGGTTGTT
CTGGATTTGGATACCTTTCAAATGCTGATTGCCAAATTCTTAGACGCTCAAGCCAAACGTCAGGCAACCATCCAAGAAAA
TCTGCCCGTCGAATACGGACTGGAAGATGATCTAGATCGCCTAGGAGGAATTGAAGATCTATCTACCAATGATGCAGATA
TCAATCTCGATGAGGCCCTTCTAGATGAGAATGCGGCACCCGTCATTTCTTTGGTCAATAAGATATTGGCAAAAGCGTTG
CAAAACGACATTTCTGATATTCATATTGAGCCGCAAGAAGACAATTTGCGCGTTCGCTTTCGTAAAGACGGCCTACTGCA
CAATGCTTTTGCACCCCTACCACGTCGCATTGCTCCCGCGTTAGTCTCTCGTTTCAAGATCATCGCTAACCTAGATATTG
CGGAACGGCGTTTACCCCAAGATGGCCGGATTCTTCGGTTATTTCAAGGCCGCAAAGTTGACTTTCGAGTCAGCACCTTA
CCCAGCCGCTATGGCGAGAAGGTGGTTTTGCGGATTTTAGACAACTCTGCCATGCAGTTGGGTCTCAACAACCTAATTGA
CGATGAAGCCAGCCTTGAGATTGTACGGGAAATGGTAGCTCGCCCGTTTGGGTTGATTTTGGTTACAGGCCCCACCGGTT
CTGGTAAAACGACGACTCTATATTCAGCCTTGACTGAGCGTAATACGCCTGAGGTGAATATCAGTACCGTTGAAGATCCC
ATTGAATATACCCTGCAGGGAATTACCCAAGTTCAAGTCCTGCGGGAAAAAGGCATGGACTTCGCATCCATTCTGCGGGC
CTTTCTGCGACAAGACCCGGATGTCCTACTTGTGGGTGAGACTCGGGACAAGGAAACTGCAAAAACGGCGATTGAGGCAG
CGTTAACTGGTCACTTGGTGTTAACGACCTTGCACACCAACGATGCAGCCAGCGCCATTGTGCGTTTGGCAGAGATGGAT
GTGGAACCGTTTATGGTCTCGGCGTCCTTATTGGGTGTGGTTGCCCAGCGACTGGTTCGTCGCCTTTGTCCAGACTGTCG
GATTCCCTATCGTCCTTCCCCAGAAAAGCTGGCTCAGTTCGGGTTAATGGCTTCCGCCAAAACGGAGGTCACCTTTTACA
AAGCAAATACCTCCACGTCAGACGATATTTTGGCAACGGCAAACACCGAAGACGCTTGCCCCACCTGTGCTGGCATTGGT
TACTCCGGGCGATGCGGGGTCTATGAAGTCATGCAGGTAACTGAAAAGTTAAAGGGACTAATTACCGAAGGAGCCCCGAC
AGAGCACATCAAAGAAGTAGCTGTCGAAGAAGGGATGCAAACCTTGCTAGCCTACAGCCTGAATCTGGTTGAAAAAGGCG
CAACGTCCCTAGAAGAGGTAGAACGAGTCACCTTCTCCGATAGTGGATTGAAAACAGAGCTAAAAGCGAAGCGCAAGACC
GCACTGAACTGTCTGGTTTGCAATGCAGGGCTCTTACCGGAGTGGTTGGACTGTCCTTACTGCCTGACGCCCAGATTTGC
AGAGCATAATCCTGCCAATCAACTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(61-178)

Type II/IV secretion system protein (T2SSE)

(234-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

59.789

98.37

0.588