Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HG267_RS33740 Genome accession   NZ_CP064879
Coordinates   8333371..8335386 (+) Length   671 a.a.
NCBI ID   WP_045874067.1    Uniprot ID   A0A0D6KDM9
Organism   Tolypothrix sp. PCC 7601     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 8328371..8340386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG267_RS33730 (HG267_33695) dnaK 8329914..8331872 (-) 1959 WP_045874065.1 molecular chaperone DnaK -
  HG267_RS33735 (HG267_33700) grpE 8332205..8332951 (-) 747 WP_045874066.1 nucleotide exchange factor GrpE -
  HG267_RS33740 (HG267_33705) pilB/pilB1 8333371..8335386 (+) 2016 WP_045874067.1 GspE/PulE family protein Machinery gene
  HG267_RS33745 (HG267_33710) pilT 8335432..8336556 (+) 1125 WP_045874068.1 type IV pilus twitching motility protein PilT Machinery gene
  HG267_RS33750 (HG267_33715) - 8336783..8338003 (+) 1221 WP_045874069.1 type II secretion system F family protein -
  HG267_RS33755 (HG267_33720) - 8338107..8338664 (+) 558 WP_045874070.1 hypothetical protein -
  HG267_RS33760 (HG267_33725) bioU 8338883..8339905 (-) 1023 WP_045874071.1 (S)-8-amino-7-oxononanoate synthase BioU -
  HG267_RS33765 (HG267_33730) - 8340158..8340352 (-) 195 WP_045874072.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75161.96 Da        Isoelectric Point: 5.1095

>NTDB_id=505591 HG267_RS33740 WP_045874067.1 8333371..8335386(+) (pilB/pilB1) [Tolypothrix sp. PCC 7601]
MTYSSPQRRSTALTTKTEFSPFGNKLVQTGYVNNEQMRQALIESRKSGTPLTEVLESITGRQLSPELVRQYKKQQLFELK
ILYGVEFLDPEVNQLANTMVGRLIETLIPVDICRRHRLIPLSKHDDQTPPCVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDELAIRQKHLEQEKFTDINQDLENLNNLDIDDAPDEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLRQAFDPLPKKIVAAVTARFKIISSLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKICLRILDNSSTQLGLNKLITDQETLDIVKDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGVNISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCPDCRIPYTPTTEELARYGMSASQEVNVTFYKANSLPNEVQADAKAKGQQVCSTCN
GVGYKGRCGVYEVMRITENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAK
RKSGLTCRTCDATLQPEWLDCPYCLTPRFTD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=505591 HG267_RS33740 WP_045874067.1 8333371..8335386(+) (pilB/pilB1) [Tolypothrix sp. PCC 7601]
ATGACTTACTCGTCACCGCAACGGCGCAGTACCGCTCTAACCACAAAAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
ACAGACTGGCTATGTTAATAACGAACAGATGAGACAGGCACTCATTGAAAGCCGCAAATCTGGCACACCTTTAACGGAAG
TACTAGAGTCAATTACCGGGCGGCAACTCTCACCTGAATTAGTTAGACAATACAAAAAACAGCAGTTATTTGAACTAAAA
ATACTATACGGGGTTGAATTCCTTGATCCGGAAGTCAACCAACTTGCTAATACGATGGTGGGGAGGTTAATTGAAACCCT
CATTCCCGTAGACATTTGTCGTCGCCATCGCTTAATCCCACTATCAAAACATGACGACCAAACCCCACCTTGCGTTTTGG
TGGCGATGGTCGATCCGGATAACCTGGAAGCTTCGGATGACCTCAACCGCATCTTGCGCCCACAAGGTTTAGCTTTGCAG
CGCATGGTAATTACCCAGGAAGATTACCAACAGCTGATTAATCAATACTTAGATGAACTAGCAATTAGGCAAAAACATCT
AGAACAAGAAAAGTTTACAGATATCAATCAAGATTTAGAAAACCTGAATAATCTTGATATAGATGATGCTCCTGATGAGA
TGGAAGCTGACTTAGGCGCAGCAATGAAGGGTGCAGAAGACGCACCAGTTATCAATCTCGTCAATAGAATTTTGGCCAAA
GCCTTGCATGAAAAGGTTTCGGATATTCATATTGAACCGCAGGAAGAAAATCTCCGGATTCGCTTTCGTAAGGATGGGGT
ACTGCGTCAAGCATTTGACCCCTTGCCGAAAAAAATCGTGGCTGCGGTGACAGCCAGGTTTAAAATCATTTCCAGCTTAG
ACATTGCTGAGAGACGTTTACCTCAAGACGGACGTATCCGGCGGATGTTTGAGGGACGTAAAGTGGATTTCCGCGTCAAC
ACCTTACCCAGTCGCTACGGGGAAAAGATTTGTCTGCGGATTTTAGATAACTCCTCTACTCAACTGGGATTGAATAAGCT
GATTACCGACCAAGAAACCTTAGATATTGTTAAGGACATGGTCAGTCGTCCCTTTGGTTTGATTCTAGTTACCGGCCCAA
CTGGCTCTGGTAAAACCACTTCGCTGTATTCAGCATTGTCAGAAAAGAACGATCCCGGAGTTAACATCAGTACAGTAGAA
GACCCAATTGAGTACAGTTTGCCAGGCATCACTCAAGTACAGGTAATTCGGGAAAAAGGACTAGATTTTGCTACCGCTTT
GCGGGCTTTTTTGCGGCAAGACCCAGATGTGTTACTCGTGGGTGAAACCAGAGACAAGGAAACTGCCAAAACAGCGATTG
AGGCAGCCTTAACAGGTCACTTAGTATTAACCACCTTACATACCAATGATGCCCCAGGTGCGATCGCACGTTTGGGAGAA
ATGGGCATTGAACCTTTCATGGTTTCTAGTTCTTTGATTGGTGTGTTGGCACAACGCTTAGTTAGGCGTGTTTGTCCTGA
TTGTCGTATTCCCTATACTCCTACAACCGAAGAACTGGCTCGTTATGGTATGTCTGCTTCTCAGGAAGTGAATGTCACTT
TCTACAAAGCTAACTCTTTGCCCAACGAAGTCCAAGCGGATGCCAAAGCTAAAGGTCAACAAGTATGCTCCACCTGTAAT
GGCGTTGGATATAAAGGACGTTGTGGTGTTTATGAAGTAATGCGAATTACCGAAAACTTGCAAACTCTCATCAACGAAGA
AGCACCAACGGAACGGATTAAGGAAGTAGCAGTAGAAGAAGGGATGAAAACCTTGCTGGCGTACAGCTTAGATTTAGTAC
GTCAAGGCTCCACCACCCTAGAAGAAGTTGAACGAGTCACCTTTACTGATACCGGTTTAGAAGCGGAATTAAAAGCTAAA
CGCAAGAGTGGTCTGACTTGCCGCACTTGTGATGCCACATTACAACCAGAATGGCTAGATTGTCCCTACTGTCTAACACC
TCGCTTTACCGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6KDM9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.889

100

0.653