Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I0K03_RS20710 Genome accession   NZ_CP064875
Coordinates   4095879..4096922 (-) Length   347 a.a.
NCBI ID   WP_001013173.1    Uniprot ID   -
Organism   Bacillus toyonensis strain P18     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4090879..4101922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0K03_RS20665 (I0K03_20665) - 4091303..4091503 (-) 201 WP_000106086.1 YqzE family protein -
  I0K03_RS20670 (I0K03_20670) - 4091542..4092039 (-) 498 WP_000836652.1 shikimate kinase -
  I0K03_RS20675 (I0K03_20675) - 4092160..4092810 (-) 651 WP_000183875.1 2OG-Fe(II) oxygenase -
  I0K03_RS20680 (I0K03_20680) comGG 4092986..4093357 (-) 372 WP_000743703.1 competence type IV pilus minor pilin ComGG -
  I0K03_RS20685 (I0K03_20685) comGF 4093354..4093827 (-) 474 WP_000477948.1 competence type IV pilus minor pilin ComGF -
  I0K03_RS20690 (I0K03_20690) - 4093797..4094099 (-) 303 WP_000829526.1 hypothetical protein -
  I0K03_RS20695 (I0K03_20695) comGD 4094092..4094547 (-) 456 WP_200203374.1 competence type IV pilus minor pilin ComGD -
  I0K03_RS20700 (I0K03_20700) comGC 4094544..4094843 (-) 300 WP_001178691.1 competence type IV pilus major pilin ComGC -
  I0K03_RS20705 (I0K03_20705) comGB 4094855..4095892 (-) 1038 WP_043322361.1 competence type IV pilus assembly protein ComGB -
  I0K03_RS20710 (I0K03_20710) comGA 4095879..4096922 (-) 1044 WP_001013173.1 competence type IV pilus ATPase ComGA Machinery gene
  I0K03_RS20715 (I0K03_20715) - 4097127..4097822 (+) 696 WP_000434113.1 metalloregulator ArsR/SmtB family transcription factor -
  I0K03_RS20720 (I0K03_20720) - 4097948..4098190 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  I0K03_RS20725 (I0K03_20725) - 4098298..4099692 (+) 1395 WP_001094345.1 L-cystine transporter -
  I0K03_RS20730 (I0K03_20730) - 4099755..4100147 (-) 393 WP_200203375.1 hypothetical protein -
  I0K03_RS20735 (I0K03_20735) - 4100235..4100450 (-) 216 WP_001008324.1 DUF3912 family protein -
  I0K03_RS20740 (I0K03_20740) - 4100703..4101014 (+) 312 WP_001092835.1 hypothetical protein -
  I0K03_RS20745 (I0K03_20745) - 4101050..4101538 (-) 489 WP_000764241.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39220.57 Da        Isoelectric Point: 8.5491

>NTDB_id=505558 I0K03_RS20710 WP_001013173.1 4095879..4096922(-) (comGA) [Bacillus toyonensis strain P18]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLITKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYRESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKRKTRRIVTLEDPVEKRKDDLLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSILCKSMRQVRQASVYELLDGYDLQQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=505558 I0K03_RS20710 WP_001013173.1 4095879..4096922(-) (comGA) [Bacillus toyonensis strain P18]
ATGAATGGAATTGAAATTTTTGCGAATACAATTTTGAAAGAAGCGTGTAGGGTACAAGCATCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAGCTACGTATAGGAAAAGATTTAATTACGAAACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCGCACTTTAAATTTTTGGCATCCATGGATATAGGAGAGAGGCGGAAGCCACAAAATGGTTCATTGTAT
TTACAAATTGATGGACAAGAAGTGTATTTACGTCTTTCAACACTTCCGACTGTATATCGAGAAAGTCTCGTGATTCGCCT
TCATTTACAAGCATCTGTGCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACGGCGGAAAAACTCCTCTCTTTTTTAC
GTTATTCACACGGTCTACTCGTATTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAGGAAAACACGTCGAATTGTAACACTTGAAGACCCAGTTGAAAAAAGAAAAGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTACGCCATGATCCAGATATTATATTAGTTG
GTGAAATCCGAGATGAAGAAACAGCAAAAATAGCTGTAAGAGCAAGTTTAACTGGACATTTAGTAATGACGACCCTGCAT
ACGAATGATGCGAAAGGAGCGATACTCAGATTTATGGATTATGGCATAACGAGGCAAGAAATTGAACAATCTTTATTGGC
TGTAGCTGCACAGCGACTTGTTGAATTAAAGTGTCCATTTTGCAGAGGAAAGTGCTCAATTTTATGTAAATCAATGCGGC
AAGTAAGACAAGCAAGCGTTTACGAGTTGTTAGATGGATATGATTTGCAACAAGCGATTAAAGAAGCGAACGGAGAATGT
GTTACGTACAAACATGAAACATTAGAATCTTCGATACGAAAAGGGTATGCTTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576