Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I0K03_RS19520 Genome accession   NZ_CP064875
Coordinates   3890660..3891094 (-) Length   144 a.a.
NCBI ID   WP_000811503.1    Uniprot ID   -
Organism   Bacillus toyonensis strain P18     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3885660..3896094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0K03_RS19505 (I0K03_19505) - 3886828..3887385 (+) 558 WP_000062081.1 PadR family transcriptional regulator -
  I0K03_RS19510 (I0K03_19510) metE 3887909..3890197 (+) 2289 WP_001007622.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  I0K03_RS19515 (I0K03_19515) - 3890235..3890624 (-) 390 WP_000424021.1 competence protein ComJ -
  I0K03_RS19520 (I0K03_19520) nucA/comI 3890660..3891094 (-) 435 WP_000811503.1 DNA-entry nuclease Machinery gene
  I0K03_RS19525 (I0K03_19525) - 3891333..3892082 (+) 750 WP_000387502.1 DUF3967 domain-containing protein -
  I0K03_RS19530 (I0K03_19530) kinB 3892120..3893394 (-) 1275 WP_000420362.1 sporulation sensor histidine kinase KinB -
  I0K03_RS19535 (I0K03_19535) - 3893706..3893906 (+) 201 WP_000929282.1 hypothetical protein -
  I0K03_RS19540 (I0K03_19540) - 3894025..3895188 (+) 1164 WP_000434561.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=505555 I0K03_RS19520 WP_000811503.1 3890660..3891094(-) (nucA/comI) [Bacillus toyonensis strain P18]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAGDRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=505555 I0K03_RS19520 WP_000811503.1 3890660..3891094(-) (nucA/comI) [Bacillus toyonensis strain P18]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATTGATCGTGGTGGTGCTGGAGATAGAAGGAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGCTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGGGCGCATATTGAATATATAAGTCCAGCGGATAATCGCGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549