Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IXY25_RS10950 Genome accession   NZ_CP064846
Coordinates   2268049..2268486 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain BZR 86     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2263049..2273486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXY25_RS10935 (IXY25_10805) - 2265907..2266269 (-) 363 WP_014304320.1 helix-turn-helix domain-containing protein -
  IXY25_RS10940 (IXY25_10810) hxlA 2266501..2267136 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  IXY25_RS10945 (IXY25_10815) hxlB 2267133..2267690 (+) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  IXY25_RS10950 (IXY25_10820) nucA/comI 2268049..2268486 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  IXY25_RS10955 (IXY25_10825) nin/comJ 2268508..2268906 (+) 399 WP_014304317.1 competence protein ComJ Regulator
  IXY25_RS10960 (IXY25_10830) - 2268947..2269165 (-) 219 Protein_2152 family 1 glycosylhydrolase -
  IXY25_RS10965 - 2269164..2269442 (-) 279 WP_107890409.1 family 1 glycosylhydrolase -
  IXY25_RS10970 (IXY25_10840) - 2269384..2269659 (-) 276 WP_369719109.1 YckD family protein -
  IXY25_RS10975 (IXY25_10845) - 2269776..2270210 (-) 435 WP_057080003.1 RDD family protein -
  IXY25_RS10980 (IXY25_10850) - 2270225..2271361 (-) 1137 WP_069013092.1 zinc-dependent alcohol dehydrogenase -
  IXY25_RS10985 (IXY25_10855) - 2271426..2272628 (-) 1203 WP_057080005.1 GTP-binding protein -
  IXY25_RS10990 - 2272781..2272906 (+) 126 Protein_2158 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=505288 IXY25_RS10950 WP_003156588.1 2268049..2268486(+) (nucA/comI) [Bacillus velezensis strain BZR 86]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=505288 IXY25_RS10950 WP_003156588.1 2268049..2268486(+) (nucA/comI) [Bacillus velezensis strain BZR 86]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641