Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IMX31_RS25480 Genome accession   NZ_CP064793
Coordinates   4911190..4912566 (-) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia megaterium strain IGA-FME-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4906190..4917566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMX31_RS25460 ispF 4907659..4908138 (-) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  IMX31_RS25465 ispD 4908135..4908836 (-) 702 WP_195781463.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IMX31_RS25470 - 4908862..4909959 (-) 1098 WP_195781464.1 PIN/TRAM domain-containing protein -
  IMX31_RS25475 disA 4910107..4911186 (-) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  IMX31_RS25480 radA 4911190..4912566 (-) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  IMX31_RS25485 clpC 4912690..4915131 (-) 2442 WP_195781465.1 ATP-dependent protease ATP-binding subunit ClpC -
  IMX31_RS25490 - 4915152..4916225 (-) 1074 WP_013054897.1 protein arginine kinase -
  IMX31_RS25495 - 4916229..4916771 (-) 543 WP_195781466.1 UvrB/UvrC motif-containing protein -
  IMX31_RS25500 - 4916813..4917274 (-) 462 WP_013054895.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=504819 IMX31_RS25480 WP_013054899.1 4911190..4912566(-) (radA) [Priestia megaterium strain IGA-FME-1]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=504819 IMX31_RS25480 WP_013054899.1 4911190..4912566(-) (radA) [Priestia megaterium strain IGA-FME-1]
ATGGCCAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCAGCAAAATGGATGGGGAAATGTCCCGGATG
CGGAAGCTGGAATTCTATGGTAGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTTCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGAATTTTTACACCTTCTGCAGAGTTA
AATCGTGTATTAGGAGGAGGTATTGTCAGAGGGTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCCGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAACAAA
CGAAGTTAAGAGCGGATAGGCTAGGTGTAAAAGCTGAAGAGCTTTATGTTCACGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGCATGCAGCCTGATTTTGTGGTAATTGATTCAATTCAAACCATTTACCATGCTGATGTGACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACGAATGGAATTGCCATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCAGGTCCAAGACTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGCCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGGTTTGGATCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGATCTCAGGGAGCGGCAG
GGTCGGTTGTTGTCGCTTCAATGGAGGGGACAAGACCTGTATTAGTAGAACTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACGGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TAGCCGTTAGTATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGTGAAGTACGCCGAGTATCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATCCC
GGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624