Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IMX31_RS09320 Genome accession   NZ_CP064793
Coordinates   1748492..1748914 (-) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain IGA-FME-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1743492..1753914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMX31_RS09290 fsa 1743787..1744455 (+) 669 WP_116076436.1 fructose-6-phosphate aldolase -
  IMX31_RS09295 - 1744534..1745019 (+) 486 WP_195782610.1 hypothetical protein -
  IMX31_RS09300 - 1745242..1746519 (+) 1278 WP_195782611.1 glycosyltransferase -
  IMX31_RS09305 - 1746543..1747199 (+) 657 WP_195782612.1 TetR/AcrR family transcriptional regulator -
  IMX31_RS09310 - 1747357..1747947 (+) 591 WP_195782613.1 hypothetical protein -
  IMX31_RS09315 - 1747986..1748330 (-) 345 WP_195782614.1 DUF1516 family protein -
  IMX31_RS09320 nucA/comI 1748492..1748914 (-) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  IMX31_RS09325 - 1749055..1749519 (-) 465 WP_195782615.1 FixH family protein -
  IMX31_RS09330 - 1749549..1750931 (-) 1383 WP_195782616.1 PepSY-associated TM helix domain-containing protein -
  IMX31_RS09335 - 1751092..1752078 (-) 987 WP_195782617.1 glycerophosphodiester phosphodiesterase family protein -
  IMX31_RS09340 - 1752254..1752895 (-) 642 WP_195782618.1 response regulator -
  IMX31_RS09345 - 1752896..1753717 (-) 822 WP_195782619.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=504795 IMX31_RS09320 WP_013057949.1 1748492..1748914(-) (nucA/comI) [Priestia megaterium strain IGA-FME-1]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=504795 IMX31_RS09320 WP_013057949.1 1748492..1748914(-) (nucA/comI) [Priestia megaterium strain IGA-FME-1]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCAGCTGTTTGTACAATTGACCGAGACGGGGCTGAGGAAAACCGCCGTGAATCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGAGCAGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCGGGGTCTTGGATCTCCCATCAAGTAGATGAGTTTCCGGACGGCACGA
AGGTGTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507