Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Azoinq_RS07530 Genome accession   NZ_CP064782
Coordinates   1648988..1650490 (-) Length   500 a.a.
NCBI ID   WP_216130396.1    Uniprot ID   A0A975XTH0
Organism   Azospira inquinata strain Azo-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643988..1655490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Azoinq_RS07500 (Azoinq_07490) ttcA 1644630..1645535 (+) 906 WP_216130408.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  Azoinq_RS07505 (Azoinq_07495) - 1645663..1646559 (+) 897 WP_216130406.1 adenylate/guanylate cyclase domain-containing protein -
  Azoinq_RS07510 (Azoinq_07500) - 1646675..1647028 (-) 354 WP_216130404.1 PsiF family protein -
  Azoinq_RS07515 (Azoinq_07505) hprK 1647104..1648042 (-) 939 WP_216130402.1 HPr(Ser) kinase/phosphatase -
  Azoinq_RS07520 (Azoinq_07510) ptsN 1648011..1648496 (-) 486 WP_216130400.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Azoinq_RS07525 (Azoinq_07515) hpf 1648648..1648971 (-) 324 WP_216130398.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  Azoinq_RS07530 (Azoinq_07520) rpoN/rpoN1 1648988..1650490 (-) 1503 WP_216130396.1 RNA polymerase factor sigma-54 Machinery gene
  Azoinq_RS07535 (Azoinq_07525) lptB 1650563..1651312 (-) 750 WP_216130394.1 LPS export ABC transporter ATP-binding protein -
  Azoinq_RS07540 (Azoinq_07530) recX 1651325..1651765 (-) 441 WP_216130392.1 recombination regulator RecX -
  Azoinq_RS07545 (Azoinq_07535) recA 1651758..1652792 (-) 1035 WP_216130390.1 recombinase RecA Machinery gene
  Azoinq_RS07550 (Azoinq_07540) - 1652904..1654460 (+) 1557 WP_216130388.1 PAS domain-containing protein -
  Azoinq_RS07555 (Azoinq_07545) bfr 1654506..1654994 (-) 489 WP_216130386.1 bacterioferritin -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55576.53 Da        Isoelectric Point: 4.7134

>NTDB_id=504726 Azoinq_RS07530 WP_216130396.1 1648988..1650490(-) (rpoN/rpoN1) [Azospira inquinata strain Azo-3]
MKPTLQLKLSQHLALTPQLQQSIRLLQLSTLELNQELEKYLTENPLLEREDEDFDGPSGPAAGEGSDSAAPDPAGTTPRE
TERGEDDSREHGEPGESAETGIRASDEGNALAAGEDDGWYAEGSYPSASRDEEDDSDYQDIQAASTSLRDHLAWQLGLTP
LSDRDRNLVRFLIEALDDDGYLTQPLEELADALPPELEIDLDDLLIALRHLQHFDPTGVGARSPQECLALQLEALPPEPT
RDLALQLVRQYLELLAGRDFTKLKKLLGCDDDQLRAAAHLVKSLNPRPGAQYAPLDTRYIIPDVVVRKLRNQWVASINPD
AYPRLRINRLYSDILSKHRGSSLAGQLQEARWLIKNVQQRFDTILRVSQAIVERQRQFFEHGEVAMRPLVLREIAEVLGL
HESTVSRVTTQKYMATPRGIFELKYFFGSHVATEAGGACSATAIRALIKQLIGGEDAKKPLSDSQISEILGQQGIVVARR
TIAKYREALNIPPANLRKTI

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=504726 Azoinq_RS07530 WP_216130396.1 1648988..1650490(-) (rpoN/rpoN1) [Azospira inquinata strain Azo-3]
ATGAAACCGACCCTCCAACTTAAACTTTCCCAGCATTTGGCGCTCACGCCCCAACTGCAACAATCCATCCGGTTGTTGCA
GCTGTCCACGCTGGAACTGAACCAGGAGCTGGAGAAGTACCTGACGGAAAATCCCCTCCTGGAACGGGAGGACGAGGATT
TCGACGGTCCTTCCGGGCCTGCCGCCGGGGAAGGAAGTGACAGTGCGGCCCCTGATCCGGCGGGCACCACGCCCCGGGAA
ACGGAGCGGGGCGAGGATGATAGCCGGGAGCACGGGGAGCCGGGGGAAAGCGCCGAAACGGGCATCCGTGCCAGCGACGA
GGGCAATGCCCTGGCCGCCGGGGAGGATGACGGCTGGTATGCGGAGGGCAGCTATCCTTCCGCCTCCCGGGACGAAGAAG
ACGATTCGGATTACCAGGACATCCAGGCCGCCAGCACCTCCCTGCGAGACCATCTGGCCTGGCAGTTAGGCCTCACCCCC
CTGAGTGACCGGGACCGGAATCTGGTACGCTTTCTCATTGAAGCCCTGGACGACGACGGCTACCTGACCCAGCCCCTGGA
AGAACTGGCGGACGCCCTGCCCCCGGAGCTCGAAATCGACCTGGACGACCTGCTCATCGCCCTGCGCCACCTCCAGCATT
TCGACCCCACCGGAGTGGGGGCCCGCTCTCCCCAGGAATGCCTGGCCCTGCAACTGGAAGCCCTGCCCCCGGAACCCACC
CGGGATCTGGCCCTGCAACTGGTGCGCCAGTATCTGGAACTCCTGGCCGGACGGGACTTCACCAAGCTGAAAAAGCTCCT
GGGCTGTGACGACGACCAGCTACGGGCCGCCGCCCATCTGGTGAAGAGCCTCAATCCCCGCCCCGGGGCCCAGTACGCCC
CCCTGGATACCCGCTACATTATTCCCGACGTGGTGGTGCGCAAGCTACGCAACCAGTGGGTGGCCAGCATCAACCCGGAC
GCCTATCCCCGGCTGCGCATCAACCGCCTGTATTCGGATATTCTCTCCAAACACCGGGGCTCCTCCCTGGCTGGCCAGCT
CCAAGAAGCCCGCTGGCTGATCAAGAACGTACAGCAGCGCTTTGACACCATTCTGCGGGTCTCCCAGGCCATCGTGGAAC
GCCAGCGCCAGTTCTTCGAACACGGGGAAGTGGCCATGCGCCCCCTGGTACTGCGGGAAATCGCCGAGGTGCTGGGGCTC
CACGAATCCACCGTTTCCCGGGTCACCACCCAGAAATACATGGCCACCCCCCGGGGCATTTTCGAACTCAAGTATTTCTT
CGGCAGCCACGTGGCCACGGAAGCGGGGGGCGCCTGTTCCGCCACCGCCATCCGGGCCCTGATCAAGCAGTTGATTGGCG
GGGAAGATGCGAAAAAGCCCCTTTCCGACAGCCAGATTTCCGAGATTCTCGGGCAACAGGGCATTGTGGTGGCCCGCCGG
ACCATAGCAAAATACCGGGAGGCGTTGAACATCCCGCCGGCTAACCTACGCAAGACCATCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-331)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-498)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.171

100

0.582