Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IWH25_RS13860 Genome accession   NZ_CP064781
Coordinates   2867194..2868555 (-) Length   453 a.a.
NCBI ID   WP_203386373.1    Uniprot ID   A0A974PX60
Organism   Azospira restricta strain DSM 18626     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2862194..2873555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS13845 (IWH25_13835) - 2862202..2862897 (-) 696 WP_203386370.1 response regulator transcription factor -
  IWH25_RS13850 (IWH25_13840) - 2862982..2864592 (-) 1611 WP_203386371.1 diguanylate cyclase -
  IWH25_RS13855 (IWH25_13845) - 2864685..2867180 (-) 2496 WP_203386372.1 ABC transporter permease -
  IWH25_RS13860 (IWH25_13850) radA 2867194..2868555 (-) 1362 WP_203386373.1 DNA repair protein RadA Machinery gene
  IWH25_RS13865 (IWH25_13855) - 2868606..2869094 (+) 489 WP_203386374.1 cyclic nucleotide-binding domain-containing protein -
  IWH25_RS13870 (IWH25_13860) alr 2869098..2870168 (-) 1071 WP_203386375.1 alanine racemase -
  IWH25_RS13875 (IWH25_13865) - 2870168..2871070 (-) 903 WP_203386376.1 LysR substrate-binding domain-containing protein -
  IWH25_RS13880 (IWH25_13870) lplT 2871227..2872492 (+) 1266 WP_203386377.1 lysophospholipid transporter LplT -
  IWH25_RS13885 (IWH25_13875) - 2872588..2873319 (+) 732 WP_203386378.1 zf-TFIIB domain-containing protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48223.70 Da        Isoelectric Point: 7.6500

>NTDB_id=504707 IWH25_RS13860 WP_203386373.1 2867194..2868555(-) (radA) [Azospira restricta strain DSM 18626]
MSKSKTVYTCSECGASLPKWQGQCPACNAWNTLVESVEEKASPAARFQSLAPAAKLQNLAEIEAREAERIATGIGEFDRA
LGGGLVAGGVVLIGGDPGIGKSTLLLQALSSLSEAQPVLYVSGEESGEQVALRARRLALDARRLQLLAEINLEKILATLQ
AEKPRVAVIDSIQTLWSDQLSSAPGSVAQVRECAAQLTRLAKTAGITIVLVGHVTKEGALAGPRVLEHIVDTVLYFEGDT
HSSFRLIRAFKNRFGAVNELGVFAMTDRGLKGVSNPSALFLSQHGQAVPGSCVLVTQEGTRPLLVEVQALVDQAHGNPRR
LTVGLEAQRLAMLLAVLHRHAGVVCFDQDVFVNAVGGVRISEPAADLAVLMAIISSLKNRPLPEKLIVFGEVGLAGEIRP
APRGQERLKEAAKLGFTRALIPEANRPRQAIPGLEVVAVSRVEEAVEKIRALE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=504707 IWH25_RS13860 WP_203386373.1 2867194..2868555(-) (radA) [Azospira restricta strain DSM 18626]
ATGAGCAAGTCAAAAACCGTCTATACCTGCAGCGAGTGCGGTGCCTCGCTGCCCAAGTGGCAGGGCCAGTGCCCGGCCTG
CAACGCGTGGAACACGCTGGTCGAGAGCGTCGAGGAGAAGGCCTCGCCGGCGGCGCGCTTCCAGTCGCTGGCGCCGGCGG
CGAAGCTGCAGAACCTCGCCGAGATCGAGGCGCGCGAGGCCGAGCGCATCGCCACCGGCATCGGCGAGTTCGACCGCGCA
CTCGGCGGCGGGCTGGTCGCCGGCGGCGTCGTGCTGATCGGCGGCGATCCCGGCATCGGCAAGAGCACGCTGCTGTTGCA
GGCGCTCTCGTCGCTGTCGGAAGCGCAGCCGGTGCTCTACGTCAGCGGCGAGGAGTCGGGCGAGCAGGTGGCCCTGCGCG
CGCGGCGCCTCGCGCTCGACGCGCGCCGCCTGCAGCTGCTCGCCGAGATCAACCTGGAGAAGATCCTCGCCACATTGCAG
GCGGAGAAGCCGCGCGTCGCGGTGATCGACTCGATCCAGACGCTGTGGTCCGATCAGCTCAGTTCTGCGCCGGGCTCGGT
GGCGCAGGTGCGCGAATGCGCGGCGCAGCTGACCCGGCTGGCGAAGACCGCCGGCATCACCATCGTGCTGGTCGGCCACG
TCACCAAGGAGGGCGCGCTGGCCGGCCCGCGCGTGCTCGAGCACATCGTCGACACGGTGCTCTACTTCGAGGGCGACACG
CATTCCAGCTTCCGCCTGATCCGCGCCTTCAAGAACCGCTTCGGCGCGGTGAACGAACTCGGCGTCTTCGCCATGACCGA
CCGCGGGCTGAAGGGGGTCAGCAACCCGTCGGCGCTGTTCCTGTCGCAGCACGGGCAGGCGGTGCCGGGGTCCTGCGTGC
TGGTCACGCAGGAAGGGACGCGGCCGCTCTTGGTCGAGGTGCAGGCGCTGGTCGACCAGGCGCACGGCAACCCGCGCCGG
CTGACCGTCGGCCTGGAGGCGCAGCGGCTGGCGATGCTGCTCGCCGTGCTGCACCGCCACGCCGGCGTCGTCTGCTTCGA
CCAGGACGTCTTCGTGAACGCCGTCGGCGGCGTCCGCATCAGCGAGCCGGCGGCCGACCTGGCGGTGCTGATGGCGATCA
TCTCGTCCTTGAAGAACCGGCCGCTGCCGGAGAAGCTGATCGTCTTCGGCGAGGTCGGGCTGGCCGGCGAGATCCGGCCG
GCGCCGCGCGGCCAGGAGCGGCTGAAGGAAGCCGCCAAGCTCGGCTTCACGCGCGCGCTGATCCCCGAGGCCAACCGGCC
GCGGCAGGCGATCCCGGGGCTCGAGGTGGTCGCCGTGTCGCGCGTCGAGGAGGCGGTCGAGAAGATCCGCGCGCTGGAGT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(347-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.448

100

0.494
  radA Streptococcus mitis SK321

48.018

100

0.481
  radA Streptococcus pneumoniae D39

47.682

100

0.477
  radA Streptococcus pneumoniae TIGR4

47.682

100

0.477
  radA Streptococcus pneumoniae R6

47.682

100

0.477
  radA Streptococcus pneumoniae Rx1

47.682

100

0.477
  radA Streptococcus mitis NCTC 12261

47.461

100

0.475