Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IWH25_RS01280 Genome accession   NZ_CP064781
Coordinates   272609..273577 (+) Length   322 a.a.
NCBI ID   WP_203387554.1    Uniprot ID   A0A974Y3P8
Organism   Azospira restricta strain DSM 18626     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 267609..278577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS01255 (IWH25_01255) - 268448..268708 (-) 261 WP_203387551.1 YfhL family 4Fe-4S dicluster ferredoxin -
  IWH25_RS01260 (IWH25_01260) coaD 268722..269222 (-) 501 WP_376990124.1 pantetheine-phosphate adenylyltransferase -
  IWH25_RS01265 (IWH25_01265) rsmD 269182..269802 (-) 621 WP_203387552.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  IWH25_RS01270 (IWH25_01270) - 269799..271100 (-) 1302 WP_238998969.1 M16 family metallopeptidase -
  IWH25_RS01275 (IWH25_01275) - 271115..272488 (-) 1374 WP_203387553.1 M16 family metallopeptidase -
  IWH25_RS01280 (IWH25_01280) pilA 272609..273577 (+) 969 WP_203387554.1 signal recognition particle-docking protein FtsY Machinery gene
  IWH25_RS01285 (IWH25_01285) - 273574..274227 (+) 654 WP_203387555.1 cell division ATP-binding protein FtsE -
  IWH25_RS01290 (IWH25_01290) ftsX 274224..275120 (+) 897 WP_203387556.1 permease-like cell division protein FtsX -
  IWH25_RS01295 (IWH25_01295) rpoH 275213..276058 (+) 846 WP_203387557.1 RNA polymerase sigma factor RpoH -
  IWH25_RS01300 (IWH25_01300) coxB 276725..277930 (+) 1206 WP_203387558.1 cytochrome c oxidase subunit II -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 34423.83 Da        Isoelectric Point: 8.8894

>NTDB_id=504688 IWH25_RS01280 WP_203387554.1 272609..273577(+) (pilA) [Azospira restricta strain DSM 18626]
MFGFLKKPTDNGSAAPDAAAAPSWRERLKAGLARTRAQFGGKLKSIFARGKVDDELLEELETLLLTSDIGIEATEHLLTA
LKARAKKDKLETPEAIQQALADALLELLKPLEQPLDLASHRPFIIMIAGINGAGKTTSIGKLAKYFQSQGKSVLLAAGDT
FRAAAREQLMTWGERNNVTVVAQESGDPAAVIFDAIAAAKARGIDVVLADTAGRLPTQLHLMEEIAKVRRVIKKADHSGP
HETLLVLDANIGQNALAQVKAFDKAIHVTGLVVTKLDGTAKGGALAAIARQCPKPVRFIGVGEGIDDLRPFVARDFVDAM
FE

Nucleotide


Download         Length: 969 bp        

>NTDB_id=504688 IWH25_RS01280 WP_203387554.1 272609..273577(+) (pilA) [Azospira restricta strain DSM 18626]
ATGTTTGGCTTCCTGAAGAAACCCACCGACAACGGCAGCGCCGCACCGGACGCCGCCGCTGCCCCCTCCTGGCGCGAACG
CCTGAAGGCCGGCCTCGCGCGCACGCGCGCGCAGTTCGGCGGCAAGCTGAAATCGATCTTCGCCCGCGGCAAGGTCGACG
ACGAACTGCTCGAGGAGCTGGAGACGCTGCTGTTGACCAGCGACATCGGCATCGAGGCCACCGAGCACCTGCTGACGGCG
CTGAAGGCGCGCGCGAAGAAGGACAAGCTGGAAACGCCGGAGGCGATCCAGCAGGCGCTCGCCGACGCGCTGCTCGAGCT
GCTGAAGCCGCTCGAGCAGCCGCTCGACCTCGCCTCGCACCGCCCGTTCATCATCATGATCGCCGGCATCAACGGCGCCG
GCAAGACGACCTCGATCGGCAAGCTGGCCAAGTACTTCCAGTCGCAGGGCAAATCGGTGCTGCTCGCCGCCGGCGACACC
TTCCGCGCCGCCGCGCGCGAGCAGCTGATGACCTGGGGCGAGCGCAACAACGTCACCGTCGTCGCGCAGGAATCGGGCGA
CCCGGCGGCGGTCATCTTCGACGCGATCGCGGCCGCCAAGGCGCGCGGCATCGACGTCGTGCTCGCCGACACCGCCGGCC
GGCTGCCGACGCAGCTGCACCTGATGGAGGAAATCGCCAAGGTCCGCCGCGTGATCAAGAAGGCCGACCACAGCGGCCCG
CACGAGACGCTGCTGGTGCTCGACGCGAACATCGGCCAAAACGCGCTGGCGCAGGTGAAGGCCTTCGACAAGGCGATCCA
CGTCACCGGCCTGGTCGTCACCAAGCTCGACGGCACCGCCAAGGGCGGCGCGCTGGCGGCGATCGCGCGGCAGTGCCCGA
AGCCGGTGCGCTTCATCGGCGTCGGCGAGGGCATCGACGACCTGCGCCCCTTCGTCGCCCGCGACTTCGTGGACGCGATG
TTCGAATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(122-321)

SRP54-type protein, helical bundle domain (SRP54_N)

(39-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

65.333

93.168

0.609