Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KQ220_RS18275 Genome accession   NZ_CP076709
Coordinates   3765539..3766216 (+) Length   225 a.a.
NCBI ID   WP_034173975.1    Uniprot ID   -
Organism   Escherichia coli O158:H23 strain CFIAFB20140388     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3760539..3771216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ220_RS18240 (KQ220_18240) gspG 3761019..3761456 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  KQ220_RS18245 (KQ220_18245) gspH 3761464..3761973 (+) 510 WP_001076032.1 type II secretion system minor pseudopilin GspH -
  KQ220_RS18250 (KQ220_18250) gspI 3761970..3762347 (+) 378 WP_001041741.1 type II secretion system minor pseudopilin GspI -
  KQ220_RS18255 (KQ220_18255) gspJ 3762340..3762927 (+) 588 WP_112005381.1 type II secretion system minor pseudopilin GspJ -
  KQ220_RS18260 (KQ220_18260) gspK 3762920..3763903 (+) 984 WP_112005383.1 type II secretion system minor pseudopilin GspK -
  KQ220_RS18265 (KQ220_18265) gspL 3763918..3765081 (+) 1164 WP_112005385.1 type II secretion system protein GspL -
  KQ220_RS18270 (KQ220_18270) gspM 3765078..3765539 (+) 462 WP_024230504.1 type II secretion system protein GspM -
  KQ220_RS18275 (KQ220_18275) pilD 3765539..3766216 (+) 678 WP_034173975.1 prepilin peptidase GspO Machinery gene
  KQ220_RS18280 (KQ220_18280) bfr 3766245..3766721 (-) 477 WP_000675505.1 bacterioferritin -
  KQ220_RS18285 (KQ220_18285) bfd 3766793..3766987 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KQ220_RS18290 (KQ220_18290) chiA 3767156..3769858 (-) 2703 WP_112005387.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24826.69 Da        Isoelectric Point: 7.9585

>NTDB_id=504328 KQ220_RS18275 WP_034173975.1 3765539..3766216(+) (pilD) [Escherichia coli O158:H23 strain CFIAFB20140388]
MAMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLIHFRQKKYAWHDTVPLILCVAAAIACALTPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=504328 KQ220_RS18275 WP_034173975.1 3765539..3766216(+) (pilD) [Escherichia coli O158:H23 strain CFIAFB20140388]
ATGGCAATGCTACTGCCGCTATTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGATTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGACACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCGTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

34.661

100

0.387
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378