Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KQ217_RS17550 Genome accession   NZ_CP076706
Coordinates   3623864..3624304 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli O170:H18 strain CFIAFB20120266     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3618864..3629304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ217_RS17525 (KQ217_17525) coaE 3619354..3619974 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  KQ217_RS17530 (KQ217_17530) - 3619999..3620043 (+) 45 WP_120795372.1 protein YacM -
  KQ217_RS17535 (KQ217_17535) guaC 3620199..3621242 (+) 1044 WP_001217348.1 GMP reductase -
  KQ217_RS17540 (KQ217_17540) hofC 3621277..3622479 (-) 1203 WP_000157241.1 protein transport protein HofC -
  KQ217_RS17545 (KQ217_17545) pilB 3622469..3623854 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  KQ217_RS17550 (KQ217_17550) pilA 3623864..3624304 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  KQ217_RS17555 (KQ217_17555) nadC 3624508..3625401 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KQ217_RS17560 (KQ217_17560) ampD 3625489..3626040 (+) 552 WP_000923733.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KQ217_RS17565 (KQ217_17565) ampE 3626037..3626891 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  KQ217_RS17570 (KQ217_17570) aroP 3626934..3628304 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=504259 KQ217_RS17550 WP_000360914.1 3623864..3624304(-) (pilA) [Escherichia coli O170:H18 strain CFIAFB20120266]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=504259 KQ217_RS17550 WP_000360914.1 3623864..3624304(-) (pilA) [Escherichia coli O170:H18 strain CFIAFB20120266]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363