Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KQ217_RS11740 Genome accession   NZ_CP076706
Coordinates   2390879..2392375 (-) Length   498 a.a.
NCBI ID   WP_001040639.1    Uniprot ID   B7UL15
Organism   Escherichia coli O170:H18 strain CFIAFB20120266     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2385879..2397375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ217_RS11715 (KQ217_11715) panM 2386113..2386496 (+) 384 WP_000778808.1 aspartate 1-decarboxylase autocleavage activator PanM -
  KQ217_RS11720 (KQ217_11720) livJ 2386684..2387787 (-) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  KQ217_RS11725 (KQ217_11725) rpoH 2388058..2388912 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  KQ217_RS11730 (KQ217_11730) ftsX 2389157..2390215 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  KQ217_RS11735 (KQ217_11735) ftsE 2390208..2390876 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  KQ217_RS11740 (KQ217_11740) pilA 2390879..2392375 (-) 1497 WP_001040639.1 signal recognition particle-docking protein FtsY Machinery gene
  KQ217_RS11745 (KQ217_11745) rsmD 2392525..2393121 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  KQ217_RS11750 (KQ217_11750) yhhL 2393111..2393380 (+) 270 WP_001295207.1 DUF1145 family protein -
  KQ217_RS11755 (KQ217_11755) yhhM 2393383..2393742 (-) 360 WP_000042877.1 DUF2500 domain-containing protein -
  KQ217_RS11760 (KQ217_11760) yhhN 2393883..2394509 (+) 627 WP_000964718.1 lysoplasmalogenase -
  KQ217_RS11765 (KQ217_11765) zntA 2394583..2396781 (+) 2199 WP_000106604.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  KQ217_RS11770 (KQ217_11770) tusA 2397050..2397295 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54624.41 Da        Isoelectric Point: 4.1733

>NTDB_id=504229 KQ217_RS11740 WP_001040639.1 2390879..2392375(-) (pilA) [Escherichia coli O170:H18 strain CFIAFB20120266]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=504229 KQ217_RS11740 WP_001040639.1 2390879..2392375(-) (pilA) [Escherichia coli O170:H18 strain CFIAFB20120266]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCTGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UL15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378