Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KQ221_RS01975 Genome accession   NZ_CP076704
Coordinates   423354..423821 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli O8:H28 strain BMH-17-0027     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418354..428821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ221_RS01950 (KQ221_01950) rpsG 418495..418965 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KQ221_RS01955 (KQ221_01955) fusA 419062..421176 (+) 2115 WP_000124700.1 elongation factor G -
  KQ221_RS01960 (KQ221_01960) tuf 421247..422431 (+) 1185 WP_000031783.1 elongation factor Tu -
  KQ221_RS01965 (KQ221_01965) bfd 422614..422808 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KQ221_RS01970 (KQ221_01970) bfr 422881..423357 (+) 477 WP_000675515.1 bacterioferritin -
  KQ221_RS01975 (KQ221_01975) pilD 423354..423821 (-) 468 WP_000340171.1 A24 family peptidase Machinery gene
  KQ221_RS01980 (KQ221_01980) rpsJ 424200..424511 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KQ221_RS01985 (KQ221_01985) rplC 424544..425173 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KQ221_RS01990 (KQ221_01990) rplD 425184..425789 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KQ221_RS01995 (KQ221_01995) rplW 425786..426088 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KQ221_RS02000 (KQ221_02000) rplB 426106..426927 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KQ221_RS02005 (KQ221_02005) rpsS 426944..427222 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KQ221_RS02010 (KQ221_02010) rplV 427237..427569 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KQ221_RS02015 (KQ221_02015) rpsC 427587..428288 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KQ221_RS02020 (KQ221_02020) rplP 428301..428711 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=504121 KQ221_RS01975 WP_000340171.1 423354..423821(-) (pilD) [Escherichia coli O8:H28 strain BMH-17-0027]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=504121 KQ221_RS01975 WP_000340171.1 423354..423821(-) (pilD) [Escherichia coli O8:H28 strain BMH-17-0027]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361