Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI30_RS00190 Genome accession   NZ_CP064650
Coordinates   45094..46455 (-) Length   453 a.a.
NCBI ID   WP_408020944.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG357     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 43762..76708 45094..46455 within 0


Gene organization within MGE regions


Location: 43762..76708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI30_RS00190 (HWI30_00190) ccrA 45094..46455 (-) 1362 WP_408020944.1 cassette chromosome recombinase CcrA Machinery gene
  HWI30_RS00195 (HWI30_00195) cch1 46642..48411 (-) 1770 WP_408020945.1 cassette chromosome replicative helicase -
  HWI30_RS00200 (HWI30_00200) ssb 48411..48701 (-) 291 WP_408020946.1 cassette chromosome ssDNA-binding protein -
  HWI30_RS00205 (HWI30_00205) - 48872..49942 (+) 1071 WP_271436914.1 hypothetical protein -
  HWI30_RS00210 (HWI30_00210) - 50036..51976 (+) 1941 WP_408020947.1 ATP-dependent DNA helicase -
  HWI30_RS00215 (HWI30_00215) - 52077..52541 (+) 465 WP_023604765.1 hypothetical protein -
  HWI30_RS00220 (HWI30_00225) - 53127..53765 (+) 639 WP_001033157.1 fusidic acid resistance EF-G-binding protein FusC -
  HWI30_RS00225 (HWI30_00230) - 54241..55382 (+) 1142 WP_145449897.1 IS3 family transposase -
  HWI30_RS00230 (HWI30_00235) - 55529..55957 (-) 429 WP_001011132.1 VOC family protein -
  HWI30_RS00235 (HWI30_00240) - 56037..56966 (+) 930 WP_000750310.1 YafY family protein -
  HWI30_RS00240 (HWI30_00245) - 57403..58659 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  HWI30_RS00245 (HWI30_00250) - 59074..59313 (+) 240 WP_001282728.1 hypothetical protein -
  HWI30_RS00250 (HWI30_00255) - 59345..60019 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI30_RS00255 (HWI30_00260) - 60110..61093 (-) 984 Protein_48 protein rep -
  HWI30_RS00260 (HWI30_00265) mobV 61338..62600 (-) 1263 WP_000119405.1 MobV family relaxase -
  HWI30_RS00265 (HWI30_00270) bleO 63107..63511 (-) 405 WP_001242578.1 bleomycin binding protein -
  HWI30_RS00270 (HWI30_00275) aadD1 63728..64498 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  HWI30_RS00275 (HWI30_00280) - 64691..65365 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI30_RS00280 (HWI30_00285) - 65630..66373 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  HWI30_RS00285 (HWI30_00290) - 66470..66898 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI30_RS00290 (HWI30_00295) mecA 66944..68953 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI30_RS00295 (HWI30_00300) - 69050..70036 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI30_RS00300 (HWI30_00305) - 70026..70268 (+) 243 Protein_57 type I restriction enzyme endonuclease domain-containing protein -
  HWI30_RS00305 (HWI30_00310) - 70259..71869 (-) 1611 Protein_58 IS1182 family transposase -
  HWI30_RS00310 (HWI30_00315) - 71918..72424 (-) 507 WP_001092194.1 DUF1643 domain-containing protein -
  HWI30_RS00315 (HWI30_00320) - 72439..72750 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI30_RS00320 (HWI30_00325) - 72837..73187 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI30_RS00325 (HWI30_00330) ccrB 73709..75337 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  HWI30_RS00330 (HWI30_00335) ccrA 75359..76708 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53096.17 Da        Isoelectric Point: 9.9051

>NTDB_id=504007 HWI30_RS00190 WP_408020944.1 45094..46455(-) (ccrA) [Staphylococcus epidermidis strain UMCG357]
MVEAIGYVRQSTLKQQSLATQQSLILNTAKQYGWPNVTFYDDKKSGRHTKRSGYQKMVEMITSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKKHHVTIISVNDGYFDLSKEFDRFRLNILMSLAEMESNNISEQTRNGIREKAKQGKLITTHAPFGYRYK
QGHFVVHEEEAYTVKVVYCWYLQGLGYKKISQHLDNNPKLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIIDIDT
FEEAQERRIHKQHNRSNSRNKLKRRIKCPYCQSTLTNLTIKKENYSLRYYVCPKNMNEAYHTCPFKGINAQMIETEILST
CKAYCDNQSFHDKLNKTVLKALKHQQIKHKETHLTQAQLIEKLAQNRIDVKTFKRLSASCKSEENYPTYSTYQIDDKLKY
VIKNKITLQDIAPLIDDITITQTKQLQGIYFKNSPLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=504007 HWI30_RS00190 WP_408020944.1 45094..46455(-) (ccrA) [Staphylococcus epidermidis strain UMCG357]
ATGGTAGAAGCCATTGGTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAGCAATCTCTGATTTTAAA
TACTGCTAAGCAATATGGATGGCCTAATGTAACATTTTATGATGATAAGAAAAGCGGTCGACATACGAAACGGTCAGGTT
ATCAAAAAATGGTAGAAATGATTACGTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCTTGAATCGACTACATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAAAGCATCACGTCACAATCATCAGTGTGAATGATGGTTATTTTGA
CTTATCTAAAGAATTTGACCGCTTTCGCCTAAACATACTTATGAGTCTAGCTGAAATGGAGTCTAATAATATCAGTGAAC
AGACACGAAATGGGATAAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGTTATCGTTACAAA
CAAGGTCATTTTGTTGTACATGAAGAAGAAGCTTATACAGTCAAAGTGGTCTATTGCTGGTACTTACAAGGGCTAGGCTA
CAAAAAAATTTCCCAACATTTAGATAACAACCCTAAGCTTATTCCACGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAATTATTGTGGTCGTGTCATTAATAAATATGGAACATTTAATGATATCGTTCCACCTATTATTGATATTGATACG
TTTGAAGAAGCACAAGAGCGTCGTATTCATAAACAACACAATCGATCCAATTCTAGAAATAAGTTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAAAATTACTCTCTACGTTACTACGTTTGTCCTA
AGAATATGAATGAAGCTTATCATACGTGTCCATTTAAAGGTATCAATGCACAAATGATTGAAACAGAGATCCTCAGCACT
TGTAAAGCGTATTGTGACAATCAATCATTTCATGACAAATTAAATAAGACAGTACTAAAAGCTCTAAAACACCAACAAAT
AAAGCATAAAGAAACCCATCTAACACAAGCACAATTGATTGAAAAGCTAGCTCAAAATCGTATTGATGTAAAAACGTTCA
AACGTTTATCTGCTAGTTGTAAAAGTGAAGAGAATTATCCTACTTATTCAACCTACCAAATCGATGACAAATTAAAATAT
GTCATAAAAAATAAAATAACTTTACAGGACATAGCACCTTTGATTGATGACATTACAATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAATAGTCCACTCAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTCATAT
GA

Domains

Predicted by InterproScan.

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(168-248)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

55.079

97.792

0.539
  ccrA Staphylococcus aureus N315

51.467

97.792

0.503