Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KQ260_RS00845 Genome accession   NZ_CP076689
Coordinates   163883..164323 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain S21EC     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 158883..169323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ260_RS00820 (KQ260_00815) coaE 159373..159993 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  KQ260_RS00825 (KQ260_00820) - 160018..160062 (+) 45 WP_120795372.1 protein YacM -
  KQ260_RS00830 (KQ260_00825) guaC 160218..161261 (+) 1044 WP_001217320.1 GMP reductase -
  KQ260_RS00835 (KQ260_00830) hofC 161296..162498 (-) 1203 WP_000157289.1 protein transport protein HofC -
  KQ260_RS00840 (KQ260_00835) pilB 162488..163873 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  KQ260_RS00845 (KQ260_00840) pilA 163883..164323 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  KQ260_RS00850 (KQ260_00845) nadC 164527..165420 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KQ260_RS00855 (KQ260_00850) ampD 165508..166059 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KQ260_RS00860 (KQ260_00855) ampE 166056..166910 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  KQ260_RS00865 (KQ260_00860) aroP 166953..168323 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=503746 KQ260_RS00845 WP_000360914.1 163883..164323(-) (pilA) [Escherichia coli strain S21EC]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=503746 KQ260_RS00845 WP_000360914.1 163883..164323(-) (pilA) [Escherichia coli strain S21EC]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363