Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KQ258_RS18585 Genome accession   NZ_CP076687
Coordinates   3775788..3776465 (+) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain S22EC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3770788..3781465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ258_RS18550 (KQ258_18545) gspG 3771268..3771705 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  KQ258_RS18555 (KQ258_18550) gspH 3771713..3772222 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  KQ258_RS18560 (KQ258_18555) gspI 3772219..3772596 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  KQ258_RS18565 (KQ258_18560) gspJ 3772589..3773176 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  KQ258_RS18570 (KQ258_18565) gspK 3773169..3774152 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  KQ258_RS18575 (KQ258_18570) gspL 3774167..3775330 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  KQ258_RS18580 (KQ258_18575) gspM 3775327..3775788 (+) 462 WP_001296465.1 type II secretion system protein GspM -
  KQ258_RS18585 (KQ258_18580) pilD 3775788..3776465 (+) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  KQ258_RS18590 (KQ258_18585) bfr 3776494..3776970 (-) 477 WP_000675504.1 bacterioferritin -
  KQ258_RS18595 (KQ258_18590) bfd 3777042..3777236 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KQ258_RS18600 (KQ258_18595) chiA 3777405..3780107 (-) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=503709 KQ258_RS18585 WP_000178170.1 3775788..3776465(+) (pilD) [Escherichia coli strain S22EC]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=503709 KQ258_RS18585 WP_000178170.1 3775788..3776465(+) (pilD) [Escherichia coli strain S22EC]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382