Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KQ258_RS00570 Genome accession   NZ_CP076687
Coordinates   125619..126059 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain S22EC     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120619..131059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ258_RS00545 (KQ258_00545) coaE 121109..121729 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  KQ258_RS00550 (KQ258_00550) - 121754..121798 (+) 45 WP_120795372.1 protein YacM -
  KQ258_RS00555 (KQ258_00555) guaC 121954..122997 (+) 1044 WP_001217320.1 GMP reductase -
  KQ258_RS00560 (KQ258_00560) hofC 123032..124234 (-) 1203 WP_000157289.1 protein transport protein HofC -
  KQ258_RS00565 (KQ258_00565) pilB 124224..125609 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  KQ258_RS00570 (KQ258_00570) pilA 125619..126059 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  KQ258_RS00575 (KQ258_00575) nadC 126263..127156 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KQ258_RS00580 (KQ258_00580) ampD 127244..127795 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KQ258_RS00585 (KQ258_00585) ampE 127792..128646 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  KQ258_RS00590 (KQ258_00590) aroP 128689..130059 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=503658 KQ258_RS00570 WP_000360914.1 125619..126059(-) (pilA) [Escherichia coli strain S22EC]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=503658 KQ258_RS00570 WP_000360914.1 125619..126059(-) (pilA) [Escherichia coli strain S22EC]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363