Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   HWI23_RS00370 Genome accession   NZ_CP064603
Coordinates   77345..78973 (-) Length   542 a.a.
NCBI ID   WP_063964179.1    Uniprot ID   A0A8X8K8R4
Organism   Staphylococcus epidermidis strain UMCG350     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 72345..83973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI23_RS00335 (HWI23_00330) - 73281..73541 (-) 261 Protein_64 IS5/IS1182 family transposase -
  HWI23_RS00340 (HWI23_00335) - 73600..74097 (-) 498 WP_001631030.1 GNAT family N-acetyltransferase -
  HWI23_RS00345 (HWI23_00345) - 74769..75179 (-) 411 WP_001631031.1 DUF2871 domain-containing protein -
  HWI23_RS00350 (HWI23_00350) - 75342..75562 (-) 221 Protein_67 hypothetical protein -
  HWI23_RS00355 (HWI23_00355) - 75577..76080 (-) 504 WP_001631032.1 DUF1643 domain-containing protein -
  HWI23_RS00360 (HWI23_00360) - 76096..76407 (-) 312 WP_001631033.1 DUF960 domain-containing protein -
  HWI23_RS00365 (HWI23_00365) - 76494..76844 (-) 351 WP_001631034.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI23_RS00370 (HWI23_00370) ccrB 77345..78973 (-) 1629 WP_063964179.1 cassette chromosome recombinase CcrB Machinery gene
  HWI23_RS00375 (HWI23_00375) ccrA 78970..80331 (-) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  HWI23_RS00380 (HWI23_00380) - 80518..81147 (-) 630 Protein_73 hypothetical protein -
  HWI23_RS00385 (HWI23_00385) - 81156..81494 (+) 339 Protein_74 recombinase family protein -
  HWI23_RS00390 (HWI23_00390) - 81606..81965 (-) 360 WP_001631039.1 hypothetical protein -
  HWI23_RS00395 (HWI23_00395) - 82172..82498 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62455.66 Da        Isoelectric Point: 9.5611

>NTDB_id=503439 HWI23_RS00370 WP_063964179.1 77345..78973(-) (ccrB) [Staphylococcus epidermidis strain UMCG350]
MKQLKKKRIGLYARVSTEMQTDGYSIQGQLNQLTEYCQFQGYEVVNEYTDRGISGKTMQRPELQRMLKDANEGKLDCIMV
YKTNRLARNTSDLLNIVEELYKINVEFFSLTEKIEIASSTGKLMLQILASFAEFERNTIVENVYNGQRQRAIEGYYQGNL
PLGYDKVPDSKKELMINQHEANIVKYIFDSYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYIISNPFYIGKIQFAKY
RHWSDKRRKGLNEEPIIADGKHAPIIDRNLWDKVQFKHQESRKKPQVHGKGTNLLTGIVKCPKCGAAMAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRADVLEKYVMDQILEIIKSKKVLKQLVEKVNEKSQIDVSSLNHDIAYKQSQCEE
IKAKMHTLTKTIEDSPDLDSVLKPTILSYQDELNQINNQIYQLEQDKQAEAPHYDADMIANVLQAIFKDIDKLEKSQLKS
LYLTIIDRIDIRKDEHHKKQFYVTLKLNNDIIKQLFNNHPLDEVLPSTSSLFLPQTLYITIE

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=503439 HWI23_RS00370 WP_063964179.1 77345..78973(-) (ccrB) [Staphylococcus epidermidis strain UMCG350]
ATGAAACAACTCAAAAAGAAACGTATTGGATTATATGCACGTGTATCGACTGAAATGCAAACGGATGGTTACAGTATTCA
AGGTCAATTGAATCAACTTACAGAGTATTGTCAATTTCAAGGCTATGAAGTCGTCAATGAATACACCGATCGTGGTATAT
CAGGTAAAACAATGCAACGTCCAGAATTACAACGTATGTTAAAAGATGCCAACGAAGGTAAATTAGATTGTATTATGGTC
TACAAAACAAATCGTTTAGCTAGAAATACCTCAGATTTGTTAAATATTGTCGAGGAATTGTATAAGATTAATGTCGAATT
TTTTAGTCTAACTGAAAAAATAGAAATTGCCAGTTCAACAGGTAAATTGATGCTCCAAATTTTAGCCAGCTTCGCAGAAT
TTGAACGTAATACGATTGTAGAGAATGTCTACAATGGCCAAAGACAACGTGCTATTGAAGGTTATTATCAAGGTAATTTA
CCTCTAGGCTACGATAAAGTACCTGACAGTAAAAAAGAGCTCATGATCAATCAACATGAAGCGAATATTGTTAAATATAT
TTTTGATTCATACGCTAAAGGTCATGGTTATCGTAAGATTGCGAACGCATTGAATCATAAAGGTTATGTGACTAAGAAAG
GGAAACCTTTTAGTATTTCGTCCATTACCTACATCATCTCAAACCCTTTTTACATTGGAAAGATACAGTTTGCGAAGTAT
AGACACTGGAGCGATAAAAGACGAAAAGGACTCAATGAGGAACCGATTATCGCTGATGGTAAACATGCGCCTATTATTGA
TAGAAACCTATGGGATAAAGTACAGTTTAAACATCAAGAGAGTCGCAAGAAACCACAAGTTCACGGCAAAGGAACAAACT
TACTGACTGGGATTGTAAAATGTCCGAAATGTGGTGCAGCAATGGCGGCAAGTAATACAACTAATACATTAAAAGATGGC
ACTAAGAAAAGAATCAGATATTACTCATGTAGCAATTTTAGAAATAAAGGTTCAAAAGTTTGTTCTGCCAATAGCGTTAG
AGCAGATGTACTAGAAAAATATGTTATGGATCAGATATTGGAAATTATCAAAAGCAAAAAGGTATTGAAGCAATTGGTTG
AAAAGGTAAATGAAAAAAGTCAAATAGACGTCTCTTCTTTGAACCATGATATTGCCTATAAACAGTCACAATGTGAAGAA
ATAAAAGCAAAAATGCATACATTAACGAAAACTATTGAAGACTCACCTGACTTAGATAGCGTATTAAAACCAACAATACT
TAGCTACCAAGATGAATTGAATCAAATAAATAATCAAATCTATCAACTAGAACAAGATAAACAAGCAGAAGCTCCACATT
ATGATGCTGATATGATTGCGAATGTACTACAAGCAATATTCAAAGATATAGACAAATTAGAGAAATCTCAGTTGAAATCA
CTATATCTAACAATCATCGATCGCATTGACATAAGAAAAGATGAGCATCATAAGAAGCAATTTTATGTTACGCTCAAGTT
AAATAATGATATAATCAAACAACTATTTAATAACCATCCACTCGACGAAGTGCTCCCTAGCACTTCGTCTTTATTTTTGC
CACAAACTTTATATATAACTATTGAATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(179-277)

Ogr/Delta-like zinc finger (Ogr_Delta)

(300-355)

Resolvase, N terminal domain (Resolvase)

(11-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

72.694

100

0.727