Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KP725_RS01940 Genome accession   NZ_CP076645
Coordinates   421141..421818 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain MSI001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416141..426818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP725_RS01925 (KP725_01925) chiA 417508..420201 (+) 2694 WP_072663100.1 bifunctional chitinase/lysozyme -
  KP725_RS01930 (KP725_01930) bfd 420370..420564 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KP725_RS01935 (KP725_01935) bfr 420636..421112 (+) 477 WP_000675504.1 bacterioferritin -
  KP725_RS01940 (KP725_01940) pilD 421141..421818 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KP725_RS01945 (KP725_01945) gspM 421818..422279 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KP725_RS01950 (KP725_01950) gspL 422276..423439 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KP725_RS01955 (KP725_01955) gspK 423454..424437 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KP725_RS01960 (KP725_01960) gspJ 424430..425017 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KP725_RS01965 (KP725_01965) gspI 425010..425387 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KP725_RS01970 (KP725_01970) gspH 425384..425893 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  KP725_RS01975 (KP725_01975) gspG 425901..426338 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=503296 KP725_RS01940 WP_000178161.1 421141..421818(-) (pilD) [Escherichia coli strain MSI001]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=503296 KP725_RS01940 WP_000178161.1 421141..421818(-) (pilD) [Escherichia coli strain MSI001]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378