Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI28_RS00180 Genome accession   NZ_CP064580
Coordinates   39390..40751 (-) Length   453 a.a.
NCBI ID   WP_001631036.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG355     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 37765..72428 39390..40751 within 0


Gene organization within MGE regions


Location: 37765..72428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI28_RS00175 (HWI28_00180) ccrB 37765..39393 (-) 1629 WP_001631035.1 cassette chromosome recombinase CcrB Machinery gene
  HWI28_RS00180 (HWI28_00185) ccrA 39390..40751 (-) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  HWI28_RS00185 (HWI28_00190) - 40938..41567 (-) 630 Protein_34 hypothetical protein -
  HWI28_RS00190 (HWI28_00195) - 41576..41914 (+) 339 Protein_35 recombinase family protein -
  HWI28_RS00195 (HWI28_00200) - 42026..42385 (-) 360 WP_001631039.1 hypothetical protein -
  HWI28_RS00200 (HWI28_00205) - 42592..42918 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI28_RS00205 (HWI28_00210) - 43248..45173 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -
  HWI28_RS00210 (HWI28_00215) - 46296..47246 (-) 951 Protein_39 transposase -
  HWI28_RS00215 (HWI28_00220) - 47367..47936 (+) 570 WP_002470562.1 hypothetical protein -
  HWI28_RS00220 (HWI28_00225) - 47981..49138 (+) 1158 WP_002485721.1 hypothetical protein -
  HWI28_RS00225 (HWI28_00230) - 49321..49593 (+) 273 WP_002501902.1 hypothetical protein -
  HWI28_RS00230 (HWI28_00235) - 49623..50297 (+) 675 WP_408021365.1 IS6-like element IS257 family transposase -
  HWI28_RS00235 (HWI28_00240) lspA 50325..50777 (-) 453 WP_164071529.1 signal peptidase II -
  HWI28_RS00240 (HWI28_00245) - 50937..51338 (+) 402 WP_057504659.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI28_RS00245 (HWI28_00250) - 51592..52359 (+) 768 WP_057504658.1 ZIP family metal transporter -
  HWI28_RS00250 (HWI28_00255) - 52616..55024 (+) 2409 WP_107362604.1 heavy metal translocating P-type ATPase -
  HWI28_RS00255 (HWI28_00260) - 55240..55914 (-) 675 WP_001106022.1 IS6-like element IS257 family transposase -
  HWI28_RS00260 (HWI28_00265) - 56179..56922 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  HWI28_RS00265 (HWI28_00270) - 57019..57447 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI28_RS00270 (HWI28_00275) mecA 57493..59502 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI28_RS00275 (HWI28_00280) - 59599..60585 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI28_RS00280 (HWI28_00285) - 60575..60817 (+) 243 Protein_53 type I restriction enzyme endonuclease domain-containing protein -
  HWI28_RS00285 (HWI28_00290) - 60808..62418 (-) 1611 Protein_54 IS1182 family transposase -
  HWI28_RS00290 (HWI28_00295) - 62467..62973 (-) 507 WP_001092194.1 DUF1643 domain-containing protein -
  HWI28_RS00295 (HWI28_00300) - 62988..63299 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI28_RS00300 (HWI28_00305) - 63386..63736 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI28_RS00305 (HWI28_00310) ccrB 64258..65886 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  HWI28_RS00310 (HWI28_00315) ccrA 65908..67257 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  HWI28_RS00315 (HWI28_00320) cch1 67491..69284 (-) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  HWI28_RS00320 (HWI28_00325) ssb 69284..69580 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  HWI28_RS00325 (HWI28_00330) - 69773..70819 (-) 1047 WP_001042023.1 hypothetical protein -
  HWI28_RS00330 (HWI28_00335) - 71274..72428 (+) 1155 WP_000912116.1 ATP-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53316.45 Da        Isoelectric Point: 9.8596

>NTDB_id=503252 HWI28_RS00180 WP_001631036.1 39390..40751(-) (ccrA) [Staphylococcus epidermidis strain UMCG355]
MEEAIAYVRQSTLKQQSLATQKSLIMDTAKQYGWSNVAFYDDKKTGRHTKRSGYQKMVEIITSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKKHHITIISVNDGYFDLSKEFDCFRLNILMSLAEMESNNISEQTKNGIREKAKQGKLITTHAPFGYRYR
KGCFVVQEEEAYTVKSVYRWYLQGLGYKKISQHLDSNPNLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIIDIDT
FEEAQERRIHKQLNRSNSRNKLKRRIKCPYCQSTLTNLTIKKENYSLRYYVCPKNMNDAYRTCPFKSINAPELESEVLDT
CKKYFEEQSFHKRLNNTILKVLKQQQMKHKETHLTQEQLIEKLAQNRIDVKTFKRLSASCKSEENYSTYSTYQIDDKLKY
VIKNKITLQDIAPFIDCIKITQTKQLQGIYFKNSPLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=503252 HWI28_RS00180 WP_001631036.1 39390..40751(-) (ccrA) [Staphylococcus epidermidis strain UMCG355]
ATGGAAGAAGCGATTGCTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAAAAATCGCTCATTATGGA
TACCGCTAAGCAATATGGATGGTCTAATGTGGCGTTTTATGATGATAAGAAAACAGGTCGACATACAAAACGTTCAGGTT
ATCAAAAAATGGTAGAAATAATTACGTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCTTGAACCGACTACATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAAAGCATCACATCACAATCATCAGTGTTAACGATGGATATTTTGA
TTTGTCTAAAGAGTTTGATTGCTTTCGTTTGAACATACTTATGAGTCTAGCGGAAATGGAATCAAACAATATCAGCGAAC
AAACAAAAAATGGGATTAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGCTATCGTTATAGA
AAAGGTTGTTTTGTTGTACAAGAAGAAGAAGCGTATACAGTTAAATCGGTTTATCGCTGGTACTTACAAGGTCTAGGCTA
CAAAAAGATTTCCCAACATTTGGATAGTAATCCTAACCTTATTCCTCGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAACTATTGCGGTCGTGTCATCAATAAATATGGCACGTTTAATGATATCGTTCCACCTATTATTGATATTGATACA
TTTGAAGAAGCACAAGAGCGTCGTATTCATAAACAACTCAATCGATCCAATTCTAGAAATAAGCTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAGAACTATTCTCTACGTTACTATGTTTGTCCTA
AAAATATGAATGACGCTTATCGTACATGCCCATTTAAAAGTATCAATGCACCAGAACTTGAAAGTGAAGTGCTCGACACT
TGTAAAAAGTATTTTGAAGAACAATCGTTTCATAAACGATTGAATAACACAATACTCAAAGTCCTAAAACAGCAACAAAT
GAAGCATAAAGAAACCCATCTAACACAAGAACAACTTATCGAAAAGCTAGCTCAAAATCGTATTGATGTAAAAACGTTCA
AACGTTTATCTGCTAGTTGTAAAAGTGAAGAGAATTACTCTACTTATTCAACCTACCAAATCGATGACAAATTAAAATAT
GTCATAAAAAATAAAATAACTTTACAGGATATAGCACCTTTTATCGATTGCATCAAAATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAACAGTCCACTCAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTAATAT
GA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(168-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

54.628

97.792

0.534
  ccrA Staphylococcus aureus N315

50.564

97.792

0.494