Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KPE21_RS10345 Genome accession   NZ_CP076527
Coordinates   2114679..2115119 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain L3452210II     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2109679..2120119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPE21_RS10325 (KPE21_10325) aroP 2110677..2112050 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  KPE21_RS10330 (KPE21_10330) ampE 2112093..2112947 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KPE21_RS10335 (KPE21_10335) ampD 2112944..2113495 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KPE21_RS10340 (KPE21_10340) nadC 2113583..2114476 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KPE21_RS10345 (KPE21_10345) pilA 2114679..2115119 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  KPE21_RS10350 (KPE21_10350) pilB 2115129..2116514 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  KPE21_RS10355 (KPE21_10355) hofC 2116504..2117706 (+) 1203 WP_191636377.1 protein transport protein HofC -
  KPE21_RS10360 (KPE21_10360) guaC 2117741..2118784 (-) 1044 WP_001217342.1 GMP reductase -
  KPE21_RS10365 (KPE21_10365) - 2118940..2118984 (-) 45 WP_120795372.1 protein YacM -
  KPE21_RS10370 (KPE21_10370) coaE 2119009..2119629 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=503233 KPE21_RS10345 WP_000360895.1 2114679..2115119(+) (pilA) [Escherichia coli strain L3452210II]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=503233 KPE21_RS10345 WP_000360895.1 2114679..2115119(+) (pilA) [Escherichia coli strain L3452210II]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363