Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI33_RS00200 Genome accession   NZ_CP064563
Coordinates   46420..47769 (-) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG360     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 38005..52940 46420..47769 within 0


Gene organization within MGE regions


Location: 38005..52940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI33_RS00160 (HWI33_00160) mecA 38005..40014 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI33_RS00165 (HWI33_00165) - 40111..41097 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI33_RS00170 (HWI33_00170) - 41087..41329 (+) 243 Protein_31 type I restriction enzyme endonuclease domain-containing protein -
  HWI33_RS00175 (HWI33_00175) - 41320..42930 (-) 1611 Protein_32 IS1182 family transposase -
  HWI33_RS00180 (HWI33_00180) - 42979..43485 (-) 507 WP_001092194.1 DUF1643 domain-containing protein -
  HWI33_RS00185 (HWI33_00185) - 43500..43811 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI33_RS00190 (HWI33_00190) - 43898..44248 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI33_RS00195 (HWI33_00195) ccrB 44770..46398 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  HWI33_RS00200 (HWI33_00200) ccrA 46420..47769 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  HWI33_RS00205 (HWI33_00205) cch1 48003..49796 (-) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  HWI33_RS00210 (HWI33_00210) ssb 49796..50092 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  HWI33_RS00215 (HWI33_00215) - 50285..51331 (-) 1047 WP_001042023.1 hypothetical protein -
  HWI33_RS00220 (HWI33_00220) - 51786..52940 (+) 1155 WP_000912116.1 ATP-binding protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=503102 HWI33_RS00200 WP_000815640.1 46420..47769(-) (ccrA) [Staphylococcus epidermidis strain UMCG360]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=503102 HWI33_RS00200 WP_000815640.1 46420..47769(-) (ccrA) [Staphylococcus epidermidis strain UMCG360]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759