Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HWI43_RS10985 Genome accession   NZ_CP064528
Coordinates   2313585..2314958 (-) Length   457 a.a.
NCBI ID   WP_408020416.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG370     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2308585..2319958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI43_RS10970 (HWI43_10880) cysE 2309569..2310198 (-) 630 WP_172952487.1 serine O-acetyltransferase -
  HWI43_RS10975 (HWI43_10885) gltX 2310607..2312061 (-) 1455 WP_001832310.1 glutamate--tRNA ligase -
  HWI43_RS10980 (HWI43_10890) - 2312473..2313531 (-) 1059 WP_237645443.1 TRAM domain-containing protein -
  HWI43_RS10985 (HWI43_10895) radA 2313585..2314958 (-) 1374 WP_408020416.1 DNA repair protein RadA Machinery gene
  HWI43_RS10990 (HWI43_10900) - 2315665..2318118 (-) 2454 WP_408020417.1 ATP-dependent Clp protease ATP-binding subunit -
  HWI43_RS10995 (HWI43_10905) - 2318132..2319139 (-) 1008 WP_080035617.1 protein arginine kinase -
  HWI43_RS11000 (HWI43_10910) - 2319129..2319692 (-) 564 WP_002475503.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50241.90 Da        Isoelectric Point: 8.0215

>NTDB_id=502764 HWI43_RS10985 WP_408020416.1 2313585..2314958(-) (radA) [Staphylococcus epidermidis strain UMCG370]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTKSKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKPR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=502764 HWI43_RS10985 WP_408020416.1 2313585..2314958(-) (radA) [Staphylococcus epidermidis strain UMCG370]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGTAAGTGCCCTAACTG
CGGCGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGTGTTAAGCATGAAGCTACCCCTAGACTACTTACAAAATCAAAGGAATTTAACCGCGTT
TTAGGTGGAGGTATTGTAAGTGGTTCATTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCGACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTGGAAGAAGATTCTAGTCATCTTAATGTTTTAGCCGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTAGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCTGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACACAAAGTTTAATGAACATTGCTAAACAGATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGACAAATCGCCGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGACGAG
CATCACGCTTATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAGGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACTACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGTATTATTGTTGCGA
CAGCATCAAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCTCGCATAGAGCAACGTGTTCAAGAAGCGGCCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAACAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTAAAATATGCATTACATT
CAAAACCGAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

AAA domain (AAA_25)

(73-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.231

100

0.665
  radA Streptococcus pneumoniae Rx1

60.579

98.249

0.595
  radA Streptococcus pneumoniae D39

60.579

98.249

0.595
  radA Streptococcus pneumoniae R6

60.579

98.249

0.595
  radA Streptococcus pneumoniae TIGR4

60.579

98.249

0.595
  radA Streptococcus mitis NCTC 12261

60.579

98.249

0.595
  radA Streptococcus mitis SK321

60.579

98.249

0.595