Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI46_RS00185 Genome accession   NZ_CP064519
Coordinates   43850..45211 (-) Length   453 a.a.
NCBI ID   WP_002505024.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG373     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 42518..71565 43850..45211 within 0


Gene organization within MGE regions


Location: 42518..71565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI46_RS00185 (HWI46_00185) ccrA 43850..45211 (-) 1362 WP_002505024.1 cassette chromosome recombinase CcrA Machinery gene
  HWI46_RS00190 (HWI46_00190) - 45398..46027 (-) 630 Protein_35 hypothetical protein -
  HWI46_RS00195 (HWI46_00195) - 46036..46374 (+) 339 Protein_36 recombinase family protein -
  HWI46_RS00200 (HWI46_00200) - 46486..46845 (-) 360 WP_001631039.1 hypothetical protein -
  HWI46_RS00205 (HWI46_00205) - 47052..47378 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI46_RS00210 (HWI46_00210) - 47708..49633 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -
  HWI46_RS00215 (HWI46_00215) - 50961..52217 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  HWI46_RS00220 (HWI46_00220) - 52632..52871 (+) 240 WP_001282728.1 hypothetical protein -
  HWI46_RS00225 (HWI46_00225) - 52903..53577 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI46_RS00230 (HWI46_00230) - 53613..54002 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  HWI46_RS00235 (HWI46_00235) - 54879..55622 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  HWI46_RS00240 (HWI46_00240) - 55719..56147 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI46_RS00245 (HWI46_00245) mecA 56193..58202 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI46_RS00250 (HWI46_00250) - 58299..59285 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI46_RS00255 (HWI46_00255) - 59275..59517 (+) 243 Protein_48 type I restriction enzyme endonuclease domain-containing protein -
  HWI46_RS00260 (HWI46_00260) - 59508..61119 (-) 1612 Protein_49 IS1182 family transposase -
  HWI46_RS00265 (HWI46_00265) - 61168..61674 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  HWI46_RS00270 (HWI46_00270) - 61689..62000 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI46_RS00275 (HWI46_00275) - 62087..62437 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI46_RS00280 (HWI46_00280) ccrB 62959..64587 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  HWI46_RS00285 (HWI46_00285) ccrA 64609..65958 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  HWI46_RS00290 (HWI46_00290) cch1 66192..67979 (-) 1788 WP_131095907.1 cassette chromosome replicative helicase -
  HWI46_RS00295 (HWI46_00295) ssb 67979..68269 (-) 291 WP_001036438.1 cassette chromosome ssDNA-binding protein -
  HWI46_RS00300 (HWI46_00300) - 68408..69457 (+) 1050 WP_000613248.1 hypothetical protein -
  HWI46_RS00305 (HWI46_00305) - 70075..71565 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53110.20 Da        Isoelectric Point: 9.9051

>NTDB_id=502622 HWI46_RS00185 WP_002505024.1 43850..45211(-) (ccrA) [Staphylococcus epidermidis strain UMCG373]
MVEAIGYVRQSTLKQQSLATQQSLILNTAKQYGWPNVTFYDDKKSGRHTKRSGYQKMVEMITSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKKHHVTIISVNDGYFDLSKEFDRFRLNILMSLAEMESNNISEQTRNGIREKAKQGKLITTHAPFGYRYK
QGHFVVHEEEAYTVKVVYCWYLQGLGYKKISQHLDNNPKLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIIDIDT
FEEAQERRIHKQHNRSNSRNKLKRRIKCPYCQSTLTNLTIKKENYSLRYYVCPKNMNEAYHTCPFKGINAQMIETEILST
CKAYCDNQSFHDKLNKTVLKALKHQQIKHKETHLTQAQLIEKLAQNRIDVKTFKRLSASCKSEENYPTYSTYQIDDKLKY
VIKNKITLQDIAPLIDEITITQTKQLQGIYFKNSPLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=502622 HWI46_RS00185 WP_002505024.1 43850..45211(-) (ccrA) [Staphylococcus epidermidis strain UMCG373]
ATGGTAGAAGCCATTGGTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAGCAATCTCTGATTTTAAA
TACTGCTAAGCAATATGGATGGCCTAATGTAACATTTTATGATGATAAGAAAAGCGGTCGACATACGAAACGGTCAGGTT
ATCAAAAAATGGTAGAAATGATTACGTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCTTGAATCGACTACATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAAAGCATCACGTCACAATCATCAGTGTGAATGATGGTTATTTTGA
CTTATCTAAAGAATTTGACCGCTTTCGCCTAAACATACTTATGAGTCTAGCTGAAATGGAGTCTAATAATATCAGTGAAC
AGACACGAAATGGGATAAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGTTATCGTTACAAA
CAAGGTCATTTTGTTGTACATGAAGAAGAAGCTTATACAGTCAAAGTGGTCTATTGCTGGTACTTACAAGGGCTAGGCTA
CAAAAAAATTTCCCAACATTTAGATAACAACCCTAAGCTTATTCCACGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAATTATTGTGGTCGTGTCATTAATAAATATGGAACATTTAATGATATCGTTCCACCTATTATTGATATTGATACG
TTTGAAGAAGCACAAGAGCGTCGTATTCATAAACAACACAATCGATCCAATTCTAGAAATAAGTTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAAAATTACTCTCTACGTTACTACGTTTGTCCTA
AGAATATGAATGAAGCTTATCATACGTGTCCATTTAAAGGTATCAATGCACAAATGATTGAAACAGAGATCCTCAGCACT
TGTAAAGCTTATTGTGACAATCAATCATTTCATGACAAATTAAATAAGACAGTACTAAAAGCTCTAAAACACCAACAAAT
AAAGCATAAAGAAACCCATCTAACACAAGCACAATTGATTGAAAAGCTAGCTCAAAATCGTATTGATGTAAAAACGTTCA
AACGTTTATCTGCTAGTTGTAAAAGTGAAGAGAATTATCCTACTTATTCAACCTACCAAATCGATGACAAATTAAAATAT
GTCATAAAAAATAAAATAACTTTACAGGACATAGCACCTTTGATTGATGAAATTACAATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAATAGTCCACTCAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTCATAT
GA

Domains

Predicted by InterproScan.

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(168-248)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

55.305

97.792

0.541
  ccrA Staphylococcus aureus N315

51.467

97.792

0.503