Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HWI50_RS06165 Genome accession   NZ_CP064504
Coordinates   1295246..1296220 (+) Length   324 a.a.
NCBI ID   WP_002446534.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG377     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1290246..1301220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI50_RS06130 (HWI50_06120) rpmG 1290728..1290877 (+) 150 WP_001830957.1 50S ribosomal protein L33 -
  HWI50_RS06135 (HWI50_06125) - 1291058..1291609 (+) 552 WP_408020740.1 5-formyltetrahydrofolate cyclo-ligase -
  HWI50_RS06140 (HWI50_06130) - 1291610..1293070 (+) 1461 WP_145334441.1 rhomboid family intramembrane serine protease -
  HWI50_RS06145 (HWI50_06135) - 1293054..1293254 (+) 201 WP_002446537.1 YqgQ family protein -
  HWI50_RS06150 (HWI50_06140) - 1293254..1294240 (+) 987 WP_002446536.1 ROK family glucokinase -
  HWI50_RS06155 (HWI50_06145) - 1294240..1294566 (+) 327 WP_001831234.1 MTH1187 family thiamine-binding protein -
  HWI50_RS06160 (HWI50_06150) - 1294566..1295189 (+) 624 WP_002446535.1 MBL fold metallo-hydrolase -
  HWI50_RS06165 (HWI50_06155) comGA 1295246..1296220 (+) 975 WP_002446534.1 competence type IV pilus ATPase ComGA Machinery gene
  HWI50_RS06170 (HWI50_06160) comGB 1296192..1297259 (+) 1068 WP_408020299.1 competence type IV pilus assembly protein ComGB Machinery gene
  HWI50_RS06175 (HWI50_06165) comGC 1297277..1297594 (+) 318 WP_002446532.1 competence type IV pilus major pilin ComGC Machinery gene
  HWI50_RS06180 (HWI50_06170) comGD 1297584..1298021 (+) 438 WP_002446531.1 competence type IV pilus minor pilin ComGD -
  HWI50_RS06185 (HWI50_06175) - 1298008..1298301 (+) 294 WP_002446530.1 hypothetical protein -
  HWI50_RS06190 (HWI50_06180) - 1298225..1298716 (+) 492 WP_002457724.1 competence type IV pilus minor pilin ComGF -
  HWI50_RS06195 (HWI50_06185) - 1298881..1299393 (+) 513 WP_002457723.1 shikimate kinase -
  HWI50_RS06200 (HWI50_06190) gcvT 1299611..1300702 (+) 1092 WP_002457722.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37261.33 Da        Isoelectric Point: 8.8257

>NTDB_id=502494 HWI50_RS06165 WP_002446534.1 1295246..1296220(+) (comGA) [Staphylococcus epidermidis strain UMCG377]
MKILFEQIINHAIKQEASDIHFIPCEEHTIIKLRIKDELTIYDTLSFPIYKKLLIYMKFQSGLDVSTQHRAQSGRYSYKM
KRLYYLRISTLPLSLGNESCVIRIVPQYFQTKRESYEFKDFKHFMKKKQGLLLFSGPTGSGKSTLMYQMVLYAHQNLNLN
VISVEDPVEQILNGITQISVNEKAGINYESSFKAILRCDPDVILIGEIRDSTVAKYVIQASLSGHLVLSTMHANDCKGAL
LRLLEMGISVQELCQAINLISNQRLITTTTNTRQLVSELMFQSQINYFFEHNHSLPKNFTKLSTHLNKMSKEGVICEEVV
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=502494 HWI50_RS06165 WP_002446534.1 1295246..1296220(+) (comGA) [Staphylococcus epidermidis strain UMCG377]
TTGAAAATTTTATTTGAACAGATTATCAATCATGCAATTAAGCAAGAAGCAAGTGACATACACTTTATACCATGTGAGGA
ACACACTATAATTAAGCTAAGAATTAAAGATGAGCTCACAATATATGATACACTTTCATTTCCAATTTATAAAAAATTAC
TTATATACATGAAGTTTCAATCTGGGTTAGATGTATCAACACAGCATAGGGCACAAAGTGGTAGATATAGTTATAAGATG
AAGCGTTTATATTATTTAAGAATCTCTACATTACCGTTATCATTAGGCAATGAAAGTTGTGTAATTCGTATAGTACCTCA
ATACTTCCAAACTAAACGAGAATCATATGAATTTAAAGATTTTAAACATTTCATGAAAAAGAAACAAGGTCTACTTTTGT
TTAGTGGGCCAACTGGCTCTGGCAAAAGTACTTTAATGTACCAAATGGTCCTTTATGCACATCAAAATTTGAATTTAAAT
GTAATATCAGTTGAAGATCCAGTAGAACAAATACTCAATGGAATCACACAAATATCAGTTAATGAAAAGGCAGGTATAAA
TTATGAAAGCTCTTTCAAAGCCATTTTAAGGTGTGATCCGGACGTAATTTTAATTGGTGAAATCAGAGATTCTACCGTAG
CTAAATACGTGATTCAAGCCAGTTTAAGTGGACATTTAGTTTTGTCGACAATGCACGCGAATGATTGCAAGGGAGCATTA
TTACGATTATTAGAAATGGGTATTTCTGTCCAAGAGTTGTGCCAGGCAATCAATTTAATTTCTAATCAAAGATTAATCAC
TACTACTACAAATACTCGTCAACTAGTATCGGAATTAATGTTTCAAAGCCAAATCAATTATTTTTTTGAACATAATCATT
CTTTACCTAAAAATTTCACAAAATTATCTACACATTTAAACAAAATGTCTAAAGAAGGTGTTATATGTGAAGAAGTTGTC
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

70.062

100

0.701
  comGA Staphylococcus aureus N315

70.062

100

0.701