Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HBHAL_RS00605 Genome accession   NC_017668
Coordinates   115306..116685 (+) Length   459 a.a.
NCBI ID   WP_014641394.1    Uniprot ID   I0JH66
Organism   Halobacillus halophilus DSM 2266     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 110306..121685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBHAL_RS00585 (HBHAL_1099) - 110553..111017 (+) 465 WP_014641390.1 CtsR family transcriptional regulator -
  HBHAL_RS00590 (HBHAL_1100) - 111191..111586 (+) 396 WP_317615980.1 UvrB/UvrC motif-containing protein -
  HBHAL_RS00595 (HBHAL_1101) - 111586..112656 (+) 1071 WP_014641392.1 protein arginine kinase -
  HBHAL_RS00600 (HBHAL_1102) clpC 112676..115135 (+) 2460 WP_014641393.1 ATP-dependent protease ATP-binding subunit ClpC -
  HBHAL_RS00605 (HBHAL_1103) radA 115306..116685 (+) 1380 WP_014641394.1 DNA repair protein RadA Machinery gene
  HBHAL_RS00610 (HBHAL_1104) disA 116686..117759 (+) 1074 WP_014641395.1 DNA integrity scanning diadenylate cyclase DisA -
  HBHAL_RS00615 (HBHAL_1105) - 117916..119016 (+) 1101 WP_014641396.1 PIN/TRAM domain-containing protein -
  HBHAL_RS00620 (HBHAL_1106) - 119136..120326 (+) 1191 WP_014641397.1 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50154.63 Da        Isoelectric Point: 7.3515

>NTDB_id=50244 HBHAL_RS00605 WP_014641394.1 115306..116685(+) (radA) [Halobacillus halophilus DSM 2266]
MAKRKTKFVCQECGYETAKWMGKCPGCQQWNTLVEEVSAPSTNSRHVFQTSSTSSKTKPEKINSIKSEKEPRLPTDMPEF
NRVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSAQIANKDKPVLYISGEESSRQTKLRAERLNITSDELYVLSETNLQDVI
NQIEAIEPEFVVIDSIQTIFKEDVTSAPGSVSQVRECTSQLMRIAKNKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRSVKNRFGSTHEMGIFEMKEQGLVEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVEIQSLISPTAY
GNPRRMATGLDNNRVPLLMAVLEKRAGLLLQNQDAYVKVAGGVKLDEPAIDLAVALSIASSFRDQVSKAEDVVVGEVGLT
GEVRRVARIEQRVQEAAKLGFKRVIIPKKNLDGWKPSSSIEVVGVSTVQEAMKVTLGDA

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=50244 HBHAL_RS00605 WP_014641394.1 115306..116685(+) (radA) [Halobacillus halophilus DSM 2266]
TTGGCAAAAAGAAAGACAAAGTTTGTTTGCCAGGAATGTGGTTACGAAACGGCGAAATGGATGGGCAAATGTCCAGGGTG
TCAACAATGGAATACGCTCGTTGAAGAAGTGAGCGCTCCAAGTACAAATTCCCGCCATGTGTTTCAAACTAGTTCCACTT
CCTCAAAAACGAAACCAGAAAAAATCAATTCCATAAAATCCGAAAAGGAACCGCGTCTCCCGACGGATATGCCAGAGTTT
AACCGTGTACTTGGCGGAGGAATAGTAGCCGGCTCCCTGGTTTTAATTGGCGGAGATCCGGGGATAGGTAAATCAACATT
ATTGCTTCAGGTATCGGCTCAGATTGCCAATAAAGATAAGCCTGTTCTTTATATCTCAGGTGAAGAATCTTCAAGACAAA
CTAAGTTAAGAGCCGAGCGGTTAAATATAACCTCAGATGAACTCTATGTATTATCTGAAACAAATCTGCAGGATGTAATC
AACCAGATTGAAGCAATAGAACCAGAGTTTGTCGTAATTGATTCCATTCAGACCATTTTCAAGGAAGATGTTACATCTGC
TCCAGGAAGCGTATCTCAGGTTAGAGAATGTACAAGTCAATTAATGAGAATAGCCAAAAATAAAGGTATTCCAATTTTTA
TTGTAGGGCATGTTACTAAGGAAGGTTCGATAGCGGGACCAAGACTCCTCGAGCATATGGTGGACGCCGTTTTATATTTT
GAAGGGGAAAGACACCATACGTTCCGCATACTCAGAAGTGTAAAAAACCGTTTTGGTAGTACACATGAGATGGGCATTTT
CGAAATGAAAGAACAAGGTTTAGTGGAAGTATTAAACCCATCTGAAATTTTCCTGGAAGAAAGGTCTCAAGGAGCGGCAG
GTTCCGTTGTAGTTGCATCTATGGAAGGTACACGTCCTGTACTCGTAGAAATACAGTCTCTAATATCTCCTACTGCCTAC
GGAAATCCAAGAAGAATGGCTACAGGGCTTGATAACAACCGTGTTCCATTACTCATGGCCGTTTTAGAGAAGCGGGCCGG
TCTCCTCTTACAAAACCAGGATGCTTATGTGAAAGTAGCAGGGGGAGTGAAGCTGGATGAGCCTGCGATTGATTTGGCGG
TTGCTTTAAGCATTGCTTCGAGTTTTAGAGATCAAGTTTCTAAGGCTGAAGATGTAGTGGTTGGAGAAGTCGGCCTGACA
GGAGAGGTTCGCCGTGTCGCTCGTATTGAACAGCGTGTGCAGGAAGCCGCAAAGTTAGGGTTCAAGCGTGTGATTATTCC
TAAGAAAAACCTGGATGGATGGAAGCCTTCTTCTTCGATCGAAGTGGTAGGGGTAAGTACGGTACAGGAAGCTATGAAGG
TTACATTGGGGGACGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I0JH66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

71.335

99.564

0.71
  radA Streptococcus mitis NCTC 12261

62.252

98.693

0.614
  radA Streptococcus mitis SK321

62.252

98.693

0.614
  radA Streptococcus pneumoniae Rx1

62.031

98.693

0.612
  radA Streptococcus pneumoniae D39

62.031

98.693

0.612
  radA Streptococcus pneumoniae R6

62.031

98.693

0.612
  radA Streptococcus pneumoniae TIGR4

62.031

98.693

0.612

Multiple sequence alignment