Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KM937_RS20620 Genome accession   NZ_CP076344
Coordinates   4253164..4253841 (+) Length   225 a.a.
NCBI ID   WP_167777131.1    Uniprot ID   -
Organism   Escherichia coli strain AVS0096     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4248164..4258841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM937_RS20585 (KM937_20585) gspG 4248644..4249081 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  KM937_RS20590 (KM937_20590) gspH 4249089..4249598 (+) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  KM937_RS20595 (KM937_20595) gspI 4249595..4249972 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  KM937_RS20600 (KM937_20600) gspJ 4249965..4250552 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  KM937_RS20605 (KM937_20605) gspK 4250545..4251528 (+) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  KM937_RS20610 (KM937_20610) gspL 4251543..4252706 (+) 1164 Protein_4064 type II secretion system protein GspL -
  KM937_RS20615 (KM937_20615) gspM 4252703..4253164 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  KM937_RS20620 (KM937_20620) pilD 4253164..4253841 (+) 678 WP_167777131.1 prepilin peptidase GspO Machinery gene
  KM937_RS20625 (KM937_20625) bfr 4253870..4254346 (-) 477 WP_000675504.1 bacterioferritin -
  KM937_RS20630 (KM937_20630) bfd 4254418..4254612 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KM937_RS20635 (KM937_20635) chiA 4254781..4257483 (-) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24847.71 Da        Isoelectric Point: 7.6111

>NTDB_id=502257 KM937_RS20620 WP_167777131.1 4253164..4253841(+) (pilD) [Escherichia coli strain AVS0096]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVNFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=502257 KM937_RS20620 WP_167777131.1 4253164..4253841(+) (pilD) [Escherichia coli strain AVS0096]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTAATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.721

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387