Detailed information NTG0501133

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: A7A20_RS18785
• Genome accession: NZ_CP076259
• Coordinates: 3865324..3866307 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain WY517-2
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 3860324..3871307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A7A20_RS18760 (A7A20_18675)	-	3861473..3861580 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
A7A20_RS18765 (A7A20_18680)	-	3861956..3862063 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
A7A20_RS18770 (A7A20_18685)	-	3862439..3862546 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
A7A20_RS18775 (A7A20_18690)	yhjV	3863022..3864293 (+)	1272	WP_001296805.1	aromatic amino acid transport family protein	-
A7A20_RS18780 (A7A20_18695)	dppF	3864323..3865327 (-)	1005	WP_000107018.1	dipeptide ABC transporter ATP-binding subunit DppF	-
A7A20_RS18785 (A7A20_18700)	amiE	3865324..3866307 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
A7A20_RS18790 (A7A20_18705)	dppC	3866318..3867220 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
A7A20_RS18795 (A7A20_18710)	dppB	3867230..3868249 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
A7A20_RS18800 (A7A20_18715)	dppA	3868557..3870164 (-)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=501133 A7A20_RS18785 WP_001196486.1 3865324..3866307(-) (amiE) [Escherichia coli strain WY517-2]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=501133 A7A20_RS18785 WP_001196486.1 3865324..3866307(-) (amiE) [Escherichia coli strain WY517-2]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404