Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IS178_RS02740 Genome accession   NZ_CP064354
Coordinates   582181..583512 (+) Length   443 a.a.
NCBI ID   WP_016783189.1    Uniprot ID   -
Organism   Pseudomonas fragi strain NL20W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577181..588512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS178_RS02720 (IS178_02720) pilM 579533..580459 (+) 927 WP_016783185.1 type IV pilus biogenesis protein PilM -
  IS178_RS02725 (IS178_02725) - 580459..581025 (+) 567 WP_016783186.1 PilN domain-containing protein -
  IS178_RS02730 (IS178_02730) - 581025..581645 (+) 621 WP_016783187.1 type 4a pilus biogenesis protein PilO -
  IS178_RS02735 (IS178_02735) - 581642..582169 (+) 528 WP_016783188.1 pilus assembly protein PilP -
  IS178_RS02740 (IS178_02740) pilQ 582181..583512 (+) 1332 WP_016783189.1 type IV pilus secretin PilQ Machinery gene
  IS178_RS02745 (IS178_02745) aroK 583517..584035 (+) 519 WP_029611693.1 shikimate kinase AroK -
  IS178_RS02750 (IS178_02750) aroB 584192..585292 (+) 1101 WP_016783191.1 3-dehydroquinate synthase -
  IS178_RS02755 (IS178_02755) - 585303..586874 (+) 1572 WP_016783192.1 AAA family ATPase -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 47567.58 Da        Isoelectric Point: 5.1760

>NTDB_id=500983 IS178_RS02740 WP_016783189.1 582181..583512(+) (pilQ) [Pseudomonas fragi strain NL20W]
MKRISPVCVLALWSALNSQATLAAGQAAPSSIASAQYVGEPMSLNVQNLEVRTVLQSLADFNGFNLMVSDTVKGSITLSL
QDVPWDQALALVLGSKGLGKRLEGNVLWVAPLDEIAARERPEPAPLRRELVQVNYARAADIVMLFQSVTGAQAGLEGQGT
ITVDERTNNIIAFESSERLDELRRIVAQLDVPVRQVMIEAWIVEAGVDFEKSLGVRWGGASSNSGLWSASGITQPLQSGA
APGNASAPFVDLGVSRNSSGLGIAFVTNNVLLDLELTAMEKTGNGEIISQPKVVTADKETAKILKGTQIPYQQASKTGAT
SVSFKEASLSLEVTPQITPDNSVIMAVVVTKDEPDYMSRLQDVPPIKKNEVRAKVLVKDGETIVIGGVFSKTQSKVVDKV
PFLGDLPYVGRLFHRDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=500983 IS178_RS02740 WP_016783189.1 582181..583512(+) (pilQ) [Pseudomonas fragi strain NL20W]
ATGAAAAGGATTTCACCGGTCTGCGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGGCAACACTGGCGGCCGGACAGGC
CGCGCCCTCATCCATCGCGAGTGCGCAGTATGTGGGCGAACCCATGTCCCTGAACGTGCAGAACCTTGAAGTACGCACGG
TGCTGCAATCGCTGGCGGATTTTAACGGGTTCAACCTGATGGTCAGCGATACCGTGAAAGGCAGTATCACCCTCAGTTTG
CAGGACGTGCCTTGGGATCAGGCGCTGGCTCTGGTGCTTGGCAGCAAGGGCCTGGGCAAGCGCCTTGAGGGTAATGTGCT
GTGGGTCGCGCCCCTGGACGAGATCGCCGCCCGCGAGCGGCCAGAGCCTGCGCCTTTGCGTCGGGAGCTGGTGCAGGTCA
ATTACGCCAGGGCCGCAGATATCGTCATGCTGTTCCAGTCAGTGACCGGCGCTCAGGCCGGTCTCGAGGGGCAGGGCACG
ATCACGGTAGATGAGCGCACCAATAACATCATTGCCTTTGAATCCAGTGAACGGCTGGATGAGCTGCGGCGCATCGTGGC
CCAACTGGACGTGCCGGTGCGCCAGGTGATGATTGAGGCCTGGATTGTCGAGGCGGGAGTGGACTTCGAAAAAAGCCTGG
GTGTTCGCTGGGGTGGGGCATCAAGCAACAGCGGCCTGTGGAGTGCCAGCGGCATCACCCAGCCATTGCAGTCAGGGGCT
GCGCCCGGCAACGCCAGCGCGCCTTTTGTCGACCTGGGGGTTTCGCGCAACTCATCGGGGTTGGGCATCGCCTTTGTGAC
CAACAATGTGCTGCTGGACCTGGAGCTGACGGCCATGGAAAAAACCGGTAATGGCGAAATCATCTCGCAGCCCAAGGTGG
TTACCGCAGACAAGGAAACCGCAAAAATCCTCAAAGGCACGCAAATTCCCTATCAACAGGCCAGCAAAACCGGTGCTACA
TCGGTCTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACGCCGCAAATCACCCCGGACAACAGTGTCATCATGGCCGT
CGTAGTGACCAAGGATGAGCCTGATTACATGAGCCGGTTGCAGGATGTGCCGCCCATCAAGAAGAACGAGGTCAGGGCCA
AGGTGTTGGTCAAGGATGGCGAAACCATCGTGATTGGGGGGGTGTTCTCAAAAACTCAAAGCAAAGTGGTAGATAAAGTG
CCATTTTTGGGCGATCTGCCGTATGTTGGCCGACTTTTCCATAGAGACGTTGTCCTGGAAAAAAAATCCGAACTGCTGGT
GTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(130-195)

Bacterial type II and III secretion system protein (Secretin)

(278-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

64.531

98.646

0.637
  comQ Acinetobacter baylyi ADP1

42.188

100

0.427
  pilQ Acinetobacter baumannii strain A118

39.362

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.362

100

0.418
  pilQ Vibrio campbellii strain DS40M4

40.222

100

0.409
  pilQ Legionella pneumophila strain Lp02

40.826

98.42

0.402
  pilQ Legionella pneumophila strain ERS1305867

40.596

98.42

0.4
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.529

95.937

0.379
  pilQ Vibrio cholerae strain A1552

39.529

95.937

0.379