Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IS481_RS01020 Genome accession   NZ_CP064338
Coordinates   207961..209487 (+) Length   508 a.a.
NCBI ID   WP_104357487.1    Uniprot ID   A0A2S5T596
Organism   Caldimonas thermodepolymerans strain DSM 15344     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 202961..214487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS481_RS00995 (IS481_00995) argS 203360..205045 (+) 1686 WP_104357419.1 arginine--tRNA ligase -
  IS481_RS01000 (IS481_01000) - 205113..205721 (+) 609 WP_104357420.1 SPOR domain-containing protein -
  IS481_RS01005 (IS481_01005) - 205791..206429 (+) 639 WP_104357421.1 thiol:disulfide interchange protein DsbA/DsbL -
  IS481_RS01010 (IS481_01010) lptA 206544..207167 (+) 624 WP_104357422.1 lipopolysaccharide transport periplasmic protein LptA -
  IS481_RS01015 (IS481_01015) lptB 207164..207931 (+) 768 WP_104357423.1 LPS export ABC transporter ATP-binding protein -
  IS481_RS01020 (IS481_01020) rpoN/rpoN1 207961..209487 (+) 1527 WP_104357487.1 RNA polymerase factor sigma-54 Machinery gene
  IS481_RS01025 (IS481_01025) - 209647..210870 (+) 1224 WP_104357424.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  IS481_RS01030 (IS481_01030) - 210875..211624 (-) 750 WP_104357425.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  IS481_RS01035 (IS481_01035) - 211708..213375 (-) 1668 WP_104357426.1 long-chain-fatty-acid--CoA ligase -
  IS481_RS01040 (IS481_01040) - 213788..213961 (+) 174 WP_170067460.1 hypothetical protein -

Sequence

Protein


Download         Length: 508 a.a.        Molecular weight: 56543.05 Da        Isoelectric Point: 4.5833

>NTDB_id=500844 IS481_RS01020 WP_104357487.1 207961..209487(+) (rpoN/rpoN1) [Caldimonas thermodepolymerans strain DSM 15344]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEPDEDYAAEPALAAPERPLTREERAAADAEQAG
ADSFDAAEAAPTLDTVELGATERDDWENGTERDDFDGIRETPSTRNGAVDDDDLDPIDRASPGITLQDHLRQQLLGMRLA
PEDAAAVMVLIESLDEDGYLADPLEEIAERLLDGNDDPEAREDLLERLRCALKWLQNMEPTGVGARSLSECLCLQLRAME
RSEAQIIALMICKDHLEALARRDVKKLMAATGADEELLRQAQALIVSLEPKPGRPFTRAEANVIVPDVIVQKSGRGWKVV
LNPDVMPKLRINDLYAQALKQQRSSDSGQALSARLQEARWFMKNIQQRFETIQRVSQAIVERQKNFFSHGEIAMKPLVLR
EIADELGLHESTISRVTTAKYMATPFGTFELKYFFGSSLNTDAGGNASSTAVRALIKQLVSAEDPKKPLSDSQISQLLEE
QGIQVARRTVAKYREALKIAPANLRKSI

Nucleotide


Download         Length: 1527 bp        

>NTDB_id=500844 IS481_RS01020 WP_104357487.1 207961..209487(+) (rpoN/rpoN1) [Caldimonas thermodepolymerans strain DSM 15344]
ATGAAACCCTCCCTGCAGGTCCGTCTTTCGCAGCATCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTCCACCAGGAGGTCGAGCAGATGCTCGAACAGAACCCGTTCCTCGAACCCGACGAGGACTACG
CCGCCGAACCCGCGCTGGCCGCGCCCGAGCGTCCGCTGACGCGCGAGGAGCGCGCCGCGGCCGATGCCGAACAGGCCGGC
GCCGACAGCTTCGATGCGGCCGAGGCCGCGCCCACGCTGGACACGGTCGAGCTGGGCGCCACCGAGCGCGACGACTGGGA
GAACGGCACCGAGCGCGACGACTTCGACGGCATCCGCGAGACGCCCAGCACGCGCAACGGGGCGGTCGACGACGACGACC
TCGACCCGATCGACCGCGCCTCGCCCGGCATCACGCTGCAGGACCACCTGCGCCAGCAGCTGCTCGGCATGCGCCTGGCG
CCCGAGGACGCCGCGGCGGTGATGGTGCTGATCGAGTCCCTCGACGAGGACGGCTACCTCGCCGACCCGCTGGAGGAGAT
CGCCGAGCGCCTGCTGGACGGCAACGACGATCCGGAGGCGCGCGAGGACCTGCTCGAGCGCCTGCGCTGCGCGCTCAAGT
GGCTGCAGAACATGGAGCCCACCGGCGTGGGCGCGCGCAGCCTGTCCGAGTGCCTGTGCCTGCAGCTGCGCGCGATGGAG
CGCAGCGAGGCGCAGATCATCGCGCTGATGATCTGCAAGGACCACCTCGAGGCGCTGGCGCGGCGCGACGTCAAGAAGCT
GATGGCCGCCACCGGCGCCGACGAGGAGCTGCTGCGCCAGGCGCAGGCGCTGATCGTCTCGCTGGAACCCAAGCCGGGCC
GGCCGTTCACGCGCGCCGAGGCCAACGTGATCGTGCCGGACGTGATCGTGCAGAAGTCCGGGCGCGGCTGGAAGGTGGTG
CTCAACCCGGACGTGATGCCTAAGCTGCGCATCAACGACCTGTACGCGCAGGCGCTCAAGCAGCAGCGCAGCAGCGACAG
CGGCCAGGCCCTGAGCGCGCGGCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCAGCAGCGCTTCGAGACCATCCAGC
GCGTCTCGCAGGCCATCGTCGAGCGGCAGAAGAACTTCTTCAGCCACGGCGAGATCGCGATGAAGCCGCTGGTGCTGCGC
GAGATCGCCGACGAGCTGGGCCTGCACGAATCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCGTTCGG
CACCTTCGAGCTGAAGTACTTCTTCGGCTCCTCGCTCAACACCGACGCGGGCGGCAACGCGTCGAGCACCGCGGTGCGCG
CGCTGATCAAGCAGCTGGTCAGCGCCGAGGACCCGAAGAAGCCACTGTCGGACAGCCAGATCAGCCAGCTGCTCGAGGAG
CAGGGCATCCAGGTGGCGCGCCGCACGGTGGCCAAGTACCGCGAGGCCCTGAAGATCGCGCCGGCCAATCTCCGCAAGAG
CATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-335)

Sigma-54, DNA binding domain (Sigma54_DBD)

(351-506)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S5T596

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.826

100

0.559