Detailed information NTG0500692

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: JKN68_RS01330
• Genome accession: NZ_CP076235
• Coordinates: 273452..274435 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain 9.1_Anguil
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 268452..279435

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JKN68_RS01315 (JKN68_01300)	dppA	269752..271359 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
JKN68_RS01320 (JKN68_01305)	dppB	271510..272529 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
JKN68_RS01325 (JKN68_01310)	dppC	272539..273441 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
JKN68_RS01330 (JKN68_01315)	amiE	273452..274435 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
JKN68_RS01335 (JKN68_01320)	dppF	274432..275436 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
JKN68_RS01340 (JKN68_01325)	yhjV	275466..276737 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
JKN68_RS01345 (JKN68_01330)	ldrD	277213..277320 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
JKN68_RS01350 (JKN68_01335)	bcsG	277407..279086 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
JKN68_RS01355 (JKN68_01340)	bcsF	279083..279274 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=500692 JKN68_RS01330 WP_001196486.1 273452..274435(+) (amiE) [Escherichia coli O157:H7 strain 9.1_Anguil]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=500692 JKN68_RS01330 WP_001196486.1 273452..274435(+) (amiE) [Escherichia coli O157:H7 strain 9.1_Anguil]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404