Detailed information NTG0500613

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: XE90_RS01450
• Genome accession: NZ_CP076232
• Coordinates: 295566..296549 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain 7.1_Anguil
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 290566..301549

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XE90_RS01435 (XE90_001420)	dppA	291866..293473 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
XE90_RS01440 (XE90_001425)	dppB	293624..294643 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
XE90_RS01445 (XE90_001430)	dppC	294653..295555 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
XE90_RS01450 (XE90_001435)	amiE	295566..296549 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
XE90_RS01455 (XE90_001440)	dppF	296546..297550 (+)	1005	WP_046425670.1	dipeptide ABC transporter ATP-binding subunit DppF	-
XE90_RS01460 (XE90_001445)	yhjV	297580..298851 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
XE90_RS01465 (XE90_001450)	ldrD	299327..299434 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
XE90_RS01470 (XE90_001455)	bcsG	299521..301200 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
XE90_RS01475 (XE90_001460)	bcsF	301197..301388 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=500613 XE90_RS01450 WP_001196486.1 295566..296549(+) (amiE) [Escherichia coli O157:H7 strain 7.1_Anguil]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=500613 XE90_RS01450 WP_001196486.1 295566..296549(+) (amiE) [Escherichia coli O157:H7 strain 7.1_Anguil]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404