Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ISU00_RS08375 Genome accession   NZ_CP064295
Coordinates   1949207..1950562 (+) Length   451 a.a.
NCBI ID   WP_228853608.1    Uniprot ID   -
Organism   Aegicerativicinus sediminis strain SM2-F     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1944207..1955562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISU00_RS08350 panC 1944443..1945291 (+) 849 WP_228853603.1 pantoate--beta-alanine ligase -
  ISU00_RS08355 panD 1945304..1945654 (+) 351 WP_228853604.1 aspartate 1-decarboxylase -
  ISU00_RS08360 - 1945659..1946624 (+) 966 WP_228853605.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  ISU00_RS08365 - 1946691..1947836 (+) 1146 WP_228853606.1 alpha/beta hydrolase -
  ISU00_RS08370 - 1947916..1949100 (+) 1185 WP_228853607.1 alpha/beta hydrolase-fold protein -
  ISU00_RS08375 radA 1949207..1950562 (+) 1356 WP_228853608.1 DNA repair protein RadA Machinery gene
  ISU00_RS08380 ileS 1950633..1954037 (+) 3405 WP_228853609.1 isoleucine--tRNA ligase -
  ISU00_RS08385 - 1954042..1954425 (+) 384 WP_228853610.1 TraR/DksA C4-type zinc finger protein -
  ISU00_RS08390 - 1954501..1955121 (+) 621 WP_228853611.1 lipoprotein signal peptidase -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 49915.25 Da        Isoelectric Point: 6.7468

>NTDB_id=500547 ISU00_RS08375 WP_228853608.1 1949207..1950562(+) (radA) [Aegicerativicinus sediminis strain SM2-F]
MAKTKTTFFCQNCGAQFSKWQGQCTSCRQWNTIVEEVIQKPQKSDWKSDVSNTKVSKPLKIDEIDGRLEARFPTSDNEYN
RVLGGGIVPGSVILIGGEPGIGKSTLMLQLALKFPIKTLYVSGEESQKQIKMRAERISNKNNNCFILTETNTQNIFRQIE
ELEPDILIVDSIQTLHSDYIDSSAGSISQIKECASDLIRYAKETSTPVILIGHITKDGNIAGPKILEHMVDTVLQFEGDR
NHVFRILRAQKNRFGSTSELGIYEMQGSGLREVSDPSDILLSRKEEDLSGSAVAATLEGMRPLMIEIQALVSTAVYGTPQ
RSATGFNAKRLNMLLAVLEKRAGFRLGTKDVFLNVTGGITVDDPAIDLAVIAAILSSNEDQPLPRDVCFAAEVGLSGEIR
PVQRVEQRILEAEKLGFSKIFISKYSKFHSPKEGIQIIKLGRIEDLVNHLI

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=500547 ISU00_RS08375 WP_228853608.1 1949207..1950562(+) (radA) [Aegicerativicinus sediminis strain SM2-F]
ATGGCTAAAACCAAAACAACATTTTTTTGCCAAAATTGCGGAGCCCAATTTTCTAAATGGCAGGGTCAATGTACTTCTTG
CCGTCAATGGAATACCATAGTGGAAGAGGTTATCCAAAAACCTCAAAAGTCTGATTGGAAATCTGATGTTTCCAACACAA
AGGTTTCTAAACCTTTAAAAATTGACGAAATTGATGGTAGATTGGAGGCCCGTTTTCCAACTTCCGATAACGAATATAAC
AGAGTGCTCGGAGGAGGTATTGTGCCCGGTTCAGTTATTCTGATTGGTGGAGAGCCCGGTATTGGTAAAAGTACCTTAAT
GCTGCAATTAGCTCTAAAGTTTCCGATTAAAACCCTTTATGTTTCAGGTGAGGAAAGCCAAAAGCAGATTAAAATGCGAG
CTGAACGGATTAGTAATAAAAACAATAACTGTTTTATTCTTACCGAAACAAATACTCAAAATATATTTAGACAAATTGAA
GAATTAGAGCCAGATATTCTCATAGTTGATTCAATACAGACACTTCATAGCGACTATATAGATTCCTCTGCGGGAAGCAT
TTCTCAAATAAAAGAATGTGCATCAGATTTAATTCGGTATGCTAAGGAAACATCAACTCCTGTAATACTTATTGGCCATA
TAACTAAAGATGGCAATATTGCCGGACCGAAAATATTGGAACATATGGTTGATACAGTCTTGCAGTTTGAAGGGGATCGC
AACCATGTGTTTAGGATTTTAAGGGCACAAAAAAACCGATTCGGATCTACTAGTGAATTAGGGATTTATGAAATGCAAGG
TTCTGGTTTAAGAGAAGTAAGTGACCCTAGCGATATTTTATTATCGAGGAAAGAAGAAGATTTAAGCGGAAGTGCCGTGG
CGGCAACCTTAGAAGGTATGCGGCCTTTAATGATTGAAATTCAGGCCCTGGTGAGCACTGCCGTATATGGCACGCCTCAG
AGATCGGCCACCGGATTTAATGCAAAGCGCTTAAATATGTTGCTGGCCGTTTTAGAAAAACGCGCAGGTTTTCGTTTGGG
TACTAAGGATGTTTTTCTTAATGTAACAGGAGGGATCACTGTAGACGACCCTGCAATTGACCTTGCAGTAATTGCTGCTA
TTTTATCTTCAAATGAGGATCAACCACTGCCGAGAGATGTTTGTTTTGCTGCAGAAGTTGGTCTTTCTGGTGAAATTAGA
CCTGTCCAAAGGGTTGAACAGCGAATTCTTGAGGCGGAGAAACTAGGGTTTTCAAAAATTTTCATTTCTAAATACAGTAA
ATTTCACTCCCCAAAAGAAGGTATTCAAATAATTAAACTAGGAAGGATCGAGGACCTAGTTAACCACTTGATTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(326-424)

AAA domain (AAA_25)

(74-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.337

100

0.483
  radA Streptococcus mitis NCTC 12261

47.797

100

0.481
  radA Streptococcus pneumoniae Rx1

47.797

100

0.481
  radA Streptococcus pneumoniae D39

47.797

100

0.481
  radA Streptococcus pneumoniae R6

47.797

100

0.481
  radA Streptococcus pneumoniae TIGR4

47.797

100

0.481
  radA Streptococcus mitis SK321

47.461

100

0.477