Detailed information NTG0500537

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: XF37_RS01435
• Genome accession: NZ_CP076230
• Coordinates: 289395..290378 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain Rafaela_II
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 284395..295378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XF37_RS01420 (XF37_001395)	dppA	285695..287302 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
XF37_RS01425 (XF37_001400)	dppB	287453..288472 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
XF37_RS01430 (XF37_001405)	dppC	288482..289384 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
XF37_RS01435 (XF37_001410)	amiE	289395..290378 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
XF37_RS01440 (XF37_001415)	dppF	290375..291379 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
XF37_RS01445 (XF37_001420)	yhjV	291409..292680 (-)	1272	WP_046411965.1	amino acid permease	-
XF37_RS01450 (XF37_001425)	ldrD	293156..293263 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
XF37_RS01455 (XF37_001430)	bcsG	293350..295029 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
XF37_RS01460 (XF37_001435)	bcsF	295026..295217 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=500537 XF37_RS01435 WP_001196486.1 289395..290378(+) (amiE) [Escherichia coli O157:H7 strain Rafaela_II]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=500537 XF37_RS01435 WP_001196486.1 289395..290378(+) (amiE) [Escherichia coli O157:H7 strain Rafaela_II]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404