Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IQ966_RS05570 Genome accession   NZ_CP064275
Coordinates   1028231..1029283 (-) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain B1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1023231..1034283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ966_RS05530 - 1024247..1025071 (+) 825 WP_014458232.1 YqhG family protein -
  IQ966_RS05535 - 1025095..1025280 (-) 186 WP_013059170.1 YqzE family protein -
  IQ966_RS05540 comGG 1025324..1025698 (-) 375 WP_013059171.1 competence type IV pilus minor pilin ComGG -
  IQ966_RS05545 comGF 1025688..1026125 (-) 438 WP_116742548.1 competence type IV pilus minor pilin ComGF -
  IQ966_RS05550 - 1026122..1026460 (-) 339 WP_116742547.1 type II secretion system protein -
  IQ966_RS05555 comGD 1026444..1026926 (-) 483 WP_223260330.1 competence type IV pilus minor pilin ComGD -
  IQ966_RS05560 comGC 1026868..1027179 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  IQ966_RS05565 comGB 1027194..1028162 (-) 969 WP_243549942.1 competence type IV pilus assembly protein ComGB -
  IQ966_RS05570 comGA 1028231..1029283 (-) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  IQ966_RS05575 - 1029553..1029936 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IQ966_RS05580 - 1030198..1030434 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  IQ966_RS05585 - 1030472..1031104 (-) 633 WP_208732872.1 MBL fold metallo-hydrolase -
  IQ966_RS05590 - 1031313..1031489 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  IQ966_RS05595 - 1031525..1032427 (-) 903 WP_208732871.1 M14 family metallocarboxypeptidase -
  IQ966_RS05600 - 1032539..1033507 (-) 969 WP_013059183.1 ROK family glucokinase -
  IQ966_RS05605 - 1033510..1033716 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=500494 IQ966_RS05570 WP_013059177.1 1028231..1029283(-) (comGA) [Priestia megaterium strain B1]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=500494 IQ966_RS05570 WP_013059177.1 1028231..1029283(-) (comGA) [Priestia megaterium strain B1]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTACGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCGAAGGAAATGTCTG
AACGGCTCATTTCACATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCACTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCGCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCGGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCATCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGATATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGTGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCAGCTTGTAAAAAGATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCGGTCATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGTTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586