Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IQ863_RS20975 Genome accession   NZ_CP064270
Coordinates   4060070..4061122 (+) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain N447-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4055070..4066122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ863_RS20940 - 4055637..4055843 (+) 207 WP_013059184.1 YqgQ family protein -
  IQ863_RS20945 - 4055846..4056814 (+) 969 WP_013059183.1 ROK family glucokinase -
  IQ863_RS20950 - 4056926..4057828 (+) 903 WP_115654917.1 M14 family metallocarboxypeptidase -
  IQ863_RS20955 - 4057864..4058040 (-) 177 WP_013059181.1 DUF2759 domain-containing protein -
  IQ863_RS20960 - 4058249..4058881 (+) 633 WP_014458225.1 MBL fold metallo-hydrolase -
  IQ863_RS20965 - 4058919..4059155 (-) 237 WP_013059179.1 DUF2626 domain-containing protein -
  IQ863_RS20970 - 4059417..4059800 (+) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IQ863_RS20975 comGA 4060070..4061122 (+) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  IQ863_RS20980 comGB 4061113..4062159 (+) 1047 WP_182420453.1 competence type IV pilus assembly protein ComGB -
  IQ863_RS20985 comGC 4062174..4062485 (+) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  IQ863_RS20990 comGD 4062427..4062909 (+) 483 WP_226554895.1 competence type IV pilus minor pilin ComGD -
  IQ863_RS20995 - 4062893..4063231 (+) 339 WP_028411610.1 type II secretion system protein -
  IQ863_RS21000 comGF 4063228..4063665 (+) 438 WP_182420452.1 competence type IV pilus minor pilin ComGF -
  IQ863_RS21005 comGG 4063655..4064029 (+) 375 WP_182420451.1 competence type IV pilus minor pilin ComGG -
  IQ863_RS21010 - 4064073..4064258 (+) 186 WP_013059170.1 YqzE family protein -
  IQ863_RS21015 - 4064282..4065106 (-) 825 WP_014458232.1 YqhG family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=500477 IQ863_RS20975 WP_013059177.1 4060070..4061122(+) (comGA) [Priestia megaterium strain N447-3]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=500477 IQ863_RS20975 WP_013059177.1 4060070..4061122(+) (comGA) [Priestia megaterium strain N447-3]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTACGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCGAAGGAAATGTCTG
AACGGCTCATTTCACATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCACTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCGGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCATCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGATATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGTGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCGGGAAAATGCCACCCAGCTTGTAAAAAGATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCGGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGTTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586