Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IQ863_RS16470 Genome accession   NZ_CP064270
Coordinates   3194123..3195499 (-) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia megaterium strain N447-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3189123..3200499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ863_RS16450 ispF 3190592..3191071 (-) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  IQ863_RS16455 ispD 3191068..3191769 (-) 702 WP_116741825.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IQ863_RS16460 - 3191795..3192892 (-) 1098 WP_013054901.1 PIN/TRAM domain-containing protein -
  IQ863_RS16465 disA 3193040..3194119 (-) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  IQ863_RS16470 radA 3194123..3195499 (-) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  IQ863_RS16475 clpC 3195623..3198064 (-) 2442 WP_014462041.1 ATP-dependent protease ATP-binding subunit ClpC -
  IQ863_RS16480 - 3198085..3199158 (-) 1074 WP_013054897.1 protein arginine kinase -
  IQ863_RS16485 - 3199162..3199704 (-) 543 WP_014462042.1 UvrB/UvrC motif-containing protein -
  IQ863_RS16490 - 3199746..3200207 (-) 462 WP_013054895.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=500452 IQ863_RS16470 WP_013054899.1 3194123..3195499(-) (radA) [Priestia megaterium strain N447-3]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=500452 IQ863_RS16470 WP_013054899.1 3194123..3195499(-) (radA) [Priestia megaterium strain N447-3]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCCGCAAAATGGATGGGGAAATGTCCCGGATG
CGGAAGCTGGAATTCGATGGTGGAAGAAATGGAAGAAACGAAGTCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTTCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGGATTTTTACACCTTCTGCAGAGTTA
AACCGTGTATTAGGAGGCGGTATTGTCAGAGGGTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCCGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAACAAA
CGAAGTTAAGAGCGGACAGGCTAGGTGTAAAAGCTGAAGAGCTTTATGTTCACGCAGAAACAAACTTGGAATTAATACTA
GAAGCCATCTCAAGCATGCAGCCTGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTGACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACAAATGGCATTGCCATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCGGGTCCAAGACTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGTCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGATTTGGATCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGGTCTCAGGGAGCAGCAG
GGTCGGTTGTTGTCGCTTCAATGGAGGGAACAAGACCCGTATTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACAGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TAGCCGTTAGCATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGCGAAGTACGCCGAGTGTCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATTCC
GGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624